Overview
- External ontology
- TOGO:M202 — M98-5
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M202 Original source: JCM - JCM_M209 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=209
Composition
flowchart LR
CultureMech_008618["m98_5"]
ing0_4__Na2CO3__CO2_buffer["4% Na2CO3--CO2 buffer"]
CultureMech_008618 --> ing0_4__Na2CO3__CO2_buffer
ing1_Distilled_water["Distilled water"]
CultureMech_008618 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Glycerol["Glycerol"]
CultureMech_008618 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_Cellobiose["Cellobiose"]
CultureMech_008618 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Maltose["Maltose"]
CultureMech_008618 --> ing4_Maltose
CHEBI_18167["CHEBI:18167"]
ing4_Maltose -.-> CHEBI_18167
ing5_Rumen_fluid["Rumen fluid"]
CultureMech_008618 --> ing5_Rumen_fluid
ing6_Glucose["Glucose"]
CultureMech_008618 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_008618 --> ing7_Bacto_agar__BD_Difco_
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008618 --> ing8_Yeast_extract__BD_Difco_
ing9_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_008618 --> ing9_Trypticase_peptone__BD_BBL_
ing10_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008618 --> ing10_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_7H2O -.-> CHEBI_31795
ing11_NaCl["NaCl"]
CultureMech_008618 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008618 --> ing12_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_2H2O -.-> CHEBI_86158
ing13_KH2PO4["KH2PO4"]
CultureMech_008618 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_K2HPO4["K2HPO4"]
CultureMech_008618 --> ing14_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing14_K2HPO4 -.-> CHEBI_131527
ing15__NH4_2SO4["(NH4)2SO4"]
CultureMech_008618 --> ing15__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing15__NH4_2SO4 -.-> CHEBI_62946
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 4% Na2CO3--CO2 buffer | 100 G_PER_L | Role: Buffer; Properties: Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
530.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 430.0, 100.0] |
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
0.3 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 5 |
Maltose
C12H22O11 342.297 g/mol
|
0.3 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 6 | Rumen fluid | 400 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
0.3 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 8 | Bacto agar (BD-Difco) | 20 G_PER_L | Role: Solidifying component | |
| 9 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 10 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component | |
| 11 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
1.18 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 13 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.12 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.47 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 15 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.78 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 16 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
Solutions
0.05% Resazurin solution
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Solution, Defined component
1% Hemin solution
Dose: 1 G_PER_L
Role: Growth factor; Properties: Defined component, Solution, Organic compound
25% L--Ascorbic acid solution
Dose: 1 G_PER_L
Role: Vitamin; Properties: Organic compound, Solution, Defined component
5% L--Cysteine・HCl・H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
Salts solution (see below)
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.602643Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M202 |
| 2026-01-28T19:48:09.743139Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:15.798154+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:08:43.079845Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:58.725224Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008618 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.346635+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M98-5" to "m98_5" for consistent matching |
| 2026-05-16T09:03:12.165990+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.309972+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.659298+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:25.333183+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.545791+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |