Overview
- External ontology
- TOGO:M2033 — Sulfer-Free Mineral Medium (SFMM)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2033 Original source: NBRC - NBRC_M1326 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1326
Composition
flowchart LR
CultureMech_008622["sulfer_free_mineral_medium_sfmm"]
ing0_Distilled_water["Distilled water"]
CultureMech_008622 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008622 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_008622 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008622 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_Na2HPO4["Na2HPO4"]
CultureMech_008622 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_Disodium_succinate["Disodium succinate"]
CultureMech_008622 --> ing5_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing5_Disodium_succinate -.-> CHEBI_63675
ing6_NH4NO3["NH4NO3"]
CultureMech_008622 --> ing6_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing6_NH4NO3 -.-> CHEBI_63038
ing7_Agarose__Electrophoresis_grade___if_needed_["Agarose (Electrophoresis grade, if needed)"]
CultureMech_008622 --> ing7_Agarose__Electrophoresis_grade___if_needed_
ing8_FeCl2_2H2O["FeCl2·2H2O"]
CultureMech_008622 --> ing8_FeCl2_2H2O
ing9_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008622 --> ing9_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing9_FeCl3_6H2O -.-> CHEBI_86254
ing10_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_008622 --> ing10_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing11__NH4_6MoO7O24_4H2O["(NH4)6MoO7O24·4H2O"]
CultureMech_008622 --> ing11__NH4_6MoO7O24_4H2O
ing12_KOH["KOH"]
CultureMech_008622 --> ing12_KOH
CHEBI_32035["CHEBI:32035"]
ing12_KOH -.-> CHEBI_32035
ing13_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008622 --> ing13_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_4H2O -.-> CHEBI_86368
ing14_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008622 --> ing14_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_2H2O -.-> CHEBI_86318
ing15_ZnCl2["ZnCl2"]
CultureMech_008622 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_Co_NO3_2_6H2O["Co(NO3)2·6H2O"]
CultureMech_008622 --> ing16_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing16_Co_NO3_2_6H2O -.-> CHEBI_86214
ing17_FeCl2_6H2O["FeCl2·6H2O"]
CultureMech_008622 --> ing17_FeCl2_6H2O
CHEBI_30812["CHEBI:30812"]
ing17_FeCl2_6H2O -.-> CHEBI_30812
ing18_Na2B4O7_10H2O["Na2B4O7·10H2O"]
CultureMech_008622 --> ing18_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing18_Na2B4O7_10H2O -.-> CHEBI_131366
ing19_Na_EDTA["Na-EDTA"]
CultureMech_008622 --> ing19_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing19_Na_EDTA -.-> CHEBI_64734
ing20_HCl["HCl"]
CultureMech_008622 --> ing20_HCl
CHEBI_17883["CHEBI:17883"]
ing20_HCl -.-> CHEBI_17883
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1926.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 975.0, 950.0, 1.0] |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
13.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 10.0, 3.3] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.8 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
26.0 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 10.0, 16.0] |
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
2.2 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
10 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 7 |
NH4NO3
H4N.NO3 80.043 g/mol
|
3 G_PER_L |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Agarose (Electrophoresis grade, if needed) | 5 G_PER_L | Role: Solidifying component | |
| 9 | FeCl2·2H2O | 10 G_PER_L | Role: Mineral source | |
| 10 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.102 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 12 | (NH4)6MoO7O24·4H2O | 9.25 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 13 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 14 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
2.02 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.267 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
5.17 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Co(NO3)2·6H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.248 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeCl2·6H2O
Cl2Fe 126.751 g/mol
|
4.85 G_PER_L |
CHEBI:30812
iron dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
2.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 21 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Solutions
Dimethyl sulfoxide**
Dose: 5 G_PER_L
Role: Solvating media; Properties: Organic compound, Defined component, Simple component
Modified Hunter's vitamin-free mineral base*
Dose: 20 G_PER_L
Mofified metals 44***
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.923588Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2033 |
| 2026-01-28T19:48:11.847465Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: HCl |
| 2026-03-09T08:04:19.532157+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:47.374977Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:58.789954Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008622 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.410580+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sulfer-Free Mineral Medium (SFMM)" to "sulfer_free_mineral_medium_sfmm" for consistent matching |
| 2026-05-16T09:04:32.718449+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:47.352496+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.976585+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |