Overview
- External ontology
- TOGO:M2035 — CM + YE Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2035 Original source: NBRC - NBRC_M1332 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1332
Composition
flowchart LR
CultureMech_008624["cm_ye_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008624 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008624 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008624 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_008624 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Trisodium_citrate_2H2O["Trisodium citrate·2H2O"]
CultureMech_008624 --> ing4_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing4_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing5_Agar["Agar"]
CultureMech_008624 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Yeast_extract__Difco_["Yeast extract (Difco)"]
CultureMech_008624 --> ing6_Yeast_extract__Difco_
ing7_Casamino_acids__vitamin_assay__Difco_["Casamino acids, vitamin assay (Difco)"]
CultureMech_008624 --> ing7_Casamino_acids__vitamin_assay__Difco_
ing8_FeSO4_7H2O["FeSO4 7H2O"]
CultureMech_008624 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
Trisodium citrate·2H2O
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 | Yeast extract (Difco) | 10 G_PER_L | Properties: Undefined component | |
| 8 | Casamino acids, vitamin assay (Difco) | 7.5 G_PER_L | Properties: Undefined component | |
| 9 |
FeSO4 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
4.98 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
Solutions
Fe2+ solution*
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.929994Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2035 |
| 2026-01-28T19:48:30.412861Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:50.573272+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:21.837081Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:58.813952Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008624 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.434209+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CM + YE Medium" to "cm_ye_medium" for consistent matching |
| 2026-05-16T09:03:12.200208+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.343751+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.367691+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |