leptospirillum_hh_medium

CultureMech:008627 bacterial COMPLEX LIQUID
Original name: Leptospirillum (HH) Medium

Overview

External ontology
TOGO:M2038 — Leptospirillum (HH) Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2038 Original source: NBRC - NBRC_M1336 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1336

Composition

flowchart LR
    CultureMech_008627["leptospirillum_hh_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008627 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008627 --> ing1_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_2H2O -.-> CHEBI_86158
    ing2_KH2PO4["KH2PO4"]
    CultureMech_008627 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_008627 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008627 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_H2SO4["H2SO4"]
    CultureMech_008627 --> ing5_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing5_H2SO4 -.-> CHEBI_26836
    ing6_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_008627 --> ing6_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_7H2O -.-> CHEBI_75836
    ing7_H3BO3["H3BO3"]
    CultureMech_008627 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008627 --> ing8_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing8_CoCl2_6H2O -.-> CHEBI_35696
    ing9_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008627 --> ing9_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_2H2O -.-> CHEBI_86318
    ing10_ZnCl2["ZnCl2"]
    CultureMech_008627 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_2H2O["MnCl2·2H2O"]
    CultureMech_008627 --> ing11_MnCl2_2H2O
    CHEBI_131395["CHEBI:131395"]
    ing11_MnCl2_2H2O -.-> CHEBI_131395
    ing12_Na2MoO4["Na2MoO4"]
    CultureMech_008627 --> ing12_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing12_Na2MoO4 -.-> CHEBI_75215

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
951.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 950.0, 1.0]
2 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
147 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 KH2PO4
H2O4P.K 136.084 g/mol
27 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
4 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
53 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
132 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
6 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
7 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
20 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 H3BO3
H3BO3 61.833 g/mol
31 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
9 CoCl2·6H2O
2Cl.Co 129.839 g/mol
64 G_PER_L CHEBI:35696
cobalt dichloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
67 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
11 ZnCl2
2Cl.Zn 136.296 g/mol
68 G_PER_L CHEBI:49976
zinc dichloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
12 MnCl2·2H2O
Cl2Mn.2H2O 161.874 g/mol
62 G_PER_L CHEBI:131395
manganese(II) chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
13 Na2MoO4
MoO4.2Na 205.916 g/mol
10 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
 Role: Mineral source; Properties: Defined component, Simple component, Organic compound

Solutions

Solution A*

Dose: 950 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B**

Dose: 50 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Trace element solution***

Dose: 1 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

H2SO4 Solution (0.25 N)

Dose: 50 G_PER_L

Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.935949Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2038
2026-01-28T19:48:29.166302Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: H2SO4
2026-03-09T08:04:48.396679+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:09:19.420231Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:58.840250Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008627
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.461484+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Leptospirillum (HH) Medium" to "leptospirillum_hh_medium" for consistent matching
2026-05-16T09:03:12.218188+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:35.366979+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:48.566737+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.