1_3_lb

CultureMech:008632 bacterial COMPLEX SOLID_AGAR
Original name: 1/3 LB

Overview

External ontology
TOGO:M2042 — 1/3 LB
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2042 Original source: NBRC - NBRC_M1340 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1340

Composition

flowchart LR
    CultureMech_008632["1_3_lb"]
    ing0_Peptone["Peptone"]
    CultureMech_008632 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008632 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_008632 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008632 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_008632 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 3.33 G_PER_L MICRO:0000178 (source link unavailable)
peptone
NBRC Medium 1340 lists this generic peptone digest as a nutrient source.
2 Yeast extract 1.67 G_PER_L FOODON:03315426
yeast extract
NBRC Medium 1340 lists this generic yeast extract as a nutrient source.
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
NBRC Medium 1340 lists 5 g sodium chloride.
4 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
NBRC Medium 1340 lists 15 g agar as the optional solidifying component.
5 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
NBRC Medium 1340 lists 1.0 L distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.942426Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2042
2026-01-28T19:48:25.361761Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:58.875835Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008632
2026-03-17T06:30:19.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.498887+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/3 LB" to "1_3_lb" for consistent matching
2026-06-05T07:40:15.594689+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_lb_family_score15.py RESOLVED_LB_FAMILY_SCORE15 NBRC Medium 1340 lists 3.33 g peptone, 1.67 g yeast extract, 5 g NaCl, 15 g optional agar, and 1 L distilled water, with pH unadjusted.