Overview
- External ontology
- TOGO:M2052 — NBY
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2052 Original source: NBRC - NBRC_M1352 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1352
Composition
flowchart LR
CultureMech_008643["nby"]
ing0_MgSO4["MgSO4"]
CultureMech_008643 --> ing0_MgSO4
CHEBI_32599["CHEBI:32599"]
ing0_MgSO4 -.-> CHEBI_32599
ing1_Glucose["Glucose"]
CultureMech_008643 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Distilled_water["Distilled water"]
CultureMech_008643 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_Yeast_Extract["Yeast Extract"]
CultureMech_008643 --> ing3_Yeast_Extract
ing4_KH2PO4["KH2PO4"]
CultureMech_008643 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_008643 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Agar__If_needed_["Agar (If needed)"]
CultureMech_008643 --> ing6_Agar__If_needed_
ing7_Nutrient_Broth["Nutrient Broth"]
CultureMech_008643 --> ing7_Nutrient_Broth
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4
Mg.O4S 120.368 g/mol
|
1 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
50 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Solution |
| 3 |
Distilled water
H2O 18.015 g/mol
|
949 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 4 | Yeast Extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 | Agar (If needed) | 15 G_PER_L | Role: Solidifying component | |
| 8 | Nutrient Broth | 8 G_PER_L | Properties: Undefined component, Complex component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.968270Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2052 |
| 2026-01-28T19:48:25.938769Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:58.997060Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008643 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.618458+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NBY" to "nby" for consistent matching |
| 2026-06-05T07:41:39.521695+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.495808+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |