b_cye_agar_medium

CultureMech:008646 bacterial COMPLEX SOLID_AGAR
Original name: B-CYEα agar medium

Overview

External ontology
TOGO:M2056 — B-CYEα agar medium
Applications
  • Microbial cultivation
pH
6.9
Notes
TOGO M2056 imports NBRC Medium 1357 as B-CYE alpha agar medium. NBRC 1357 lists 10 g yeast extract, 0.4 g L-Cysteine hydrochloride, 0.25 g soluble iron pyrophosphate, 2 g activated carbon, 10 g ACES, 1 g alpha-Ketoglutarate potassium, 15 g agar, 1 L distilled water, and pH 6.9. NBRC 1357 notes that the prepared E-MP96 medium is available from Eiken Chemical Co., Ltd.

Composition

flowchart LR
    CultureMech_008646["b_cye_agar_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_008646 --> ing0_Yeast_extract
    ing1_L_Cysteine_hydrochloride["L-Cysteine hydrochloride"]
    CultureMech_008646 --> ing1_L_Cysteine_hydrochloride
    CHEBI_91247["CHEBI:91247"]
    ing1_L_Cysteine_hydrochloride -.-> CHEBI_91247
    ing2_Soluble_iron_pyrophosphate["Soluble iron pyrophosphate"]
    CultureMech_008646 --> ing2_Soluble_iron_pyrophosphate
    CHEBI_132767["CHEBI:132767"]
    ing2_Soluble_iron_pyrophosphate -.-> CHEBI_132767
    ing3_Activated_carbon["Activated carbon"]
    CultureMech_008646 --> ing3_Activated_carbon
    ing4_ACES__N__2_Acetamido__2_aminoethanesulfonic_acid_["ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid)"]
    CultureMech_008646 --> ing4_ACES__N__2_Acetamido__2_aminoethanesulfonic_acid_
    CHEBI_39061["CHEBI:39061"]
    ing4_ACES__N__2_Acetamido__2_aminoethanesulfonic_acid_ -.-> CHEBI_39061
    ing5___Ketoglutarate_potassium["α-Ketoglutarate potassium"]
    CultureMech_008646 --> ing5___Ketoglutarate_potassium
    ing6_Agar["Agar"]
    CultureMech_008646 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Distilled_water["Distilled water"]
    CultureMech_008646 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Yeast extract 10.0 G_PER_L FOODON:03315426
yeast extract
 NBRC Medium 1357 lists 10.0 g/L Yeast extract.
2 L-Cysteine hydrochloride
C3H7NO2S.HCl 157.622 g/mol
0.4 G_PER_L CHEBI:91247
L-cysteine hydrochloride
 NBRC Medium 1357 lists 0.4 g/L L-Cysteine hydrochloride.
3 Soluble iron pyrophosphate
4Fe.3O7P2 745.203 g/mol
0.25 G_PER_L CHEBI:132767
ferric pyrophosphate
 NBRC Medium 1357 lists 0.25 g/L Soluble iron pyrophosphate.
4 Activated carbon 2.0 G_PER_L NBRC Medium 1357 lists 2 g/L activated carbon; this adsorbent material is retained as an opaque protective component.
5 ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid) 10.0 G_PER_L CHEBI:39061
ACES
 NBRC Medium 1357 lists 10.0 g/L ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid).
6 α-Ketoglutarate potassium 1.0 G_PER_L NBRC Medium 1357 lists 1 g/L alpha-Ketoglutarate potassium; this row is left ungrounded because ChEBI has no exact term for the potassium salt.
7 Agar 15.0 G_PER_L CHEBI:2509
agar
 NBRC Medium 1357 lists 15.0 g/L Agar.
8 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 NBRC Medium 1357 lists 1 L distilled water.

Preparation

  1. Adjust pH to 6.9.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.975456Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2056
2026-01-28T19:48:10.098870Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:59.029645Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008646
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.652522+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "B-CYEα agar medium" to "b_cye_agar_medium" for consistent matching
2026-06-05T07:40:50.199046+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2056_bcye_score15.py RESOLVED_TOGO_M2056_SCORE15 TOGO M2056 imports NBRC Medium 1357 as B-CYE alpha agar medium. NBRC 1357 lists 10 g yeast extract, 0.4 g L-Cysteine hydrochloride, 0.25 g soluble iron pyrophosphate, 2 g activated carbon, 10 g ACES, 1 g alpha-Ketoglutarate potassium, 15 g agar, 1 L distilled water, and pH 6.9. NBRC 1357 notes that the prepared E-MP96 medium is available from Eiken Chemical Co., Ltd. Corrected the imported water g/L artifact, added the NBRC pH, and marked the NBRC formula as curated.