Overview
- External ontology
- TOGO:M206 — Archaeoglobus Fulgidus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M206 Original source: JCM - JCM_M213 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=213 Original post-inoculation instruction: Pressurize inoculated bottles to 200 kPa N2-CO2 (80:20).
Composition
flowchart LR
CultureMech_008658["archaeoglobus_fulgidus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008658 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008658 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008658 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008658 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_008658 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_008658 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_008658 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_008658 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_008658 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaHCO3["NaHCO3"]
CultureMech_008658 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2・6H2O"]
CultureMech_008658 --> ing10_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing10_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing11__NH4_2Ni_SO4_2_6H2O["(NH4)2Ni(SO4)2・6H2O"]
CultureMech_008658 --> ing11__NH4_2Ni_SO4_2_6H2O
CHEBI_86149["CHEBI:86149"]
ing11__NH4_2Ni_SO4_2_6H2O -.-> CHEBI_86149
ing12_Sodium_L__lactate["Sodium L--lactate"]
CultureMech_008658 --> ing12_Sodium_L__lactate
CHEBI_232798["CHEBI:232798"]
ing12_Sodium_L__lactate -.-> CHEBI_232798
ing13_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008658 --> ing13_Yeast_extract__BD_Difco_
ing14_H2SO4["H2SO4"]
CultureMech_008658 --> ing14_H2SO4
CHEBI_26836["CHEBI:26836"]
ing14_H2SO4 -.-> CHEBI_26836
ing15_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008658 --> ing15_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing15_Carbon_dioxide_gas -.-> CHEBI_16526
ing16_Nitrogen_gas["Nitrogen gas"]
CultureMech_008658 --> ing16_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing16_Nitrogen_gas -.-> CHEBI_17997
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.99 L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component Source water batch input: 990 ml = 0.99 L. This is not a measured final volume. |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 3.45 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 18 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.14 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.25 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.14 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component Source batch input: 1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 4 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 9 |
KCl
Cl.K 74.551 g/mol
|
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.34 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source batch input: 5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 11 |
Fe(NH4)2(SO4)2・6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 2 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 12 |
(NH4)2Ni(SO4)2・6H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 2 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 13 |
Sodium L--lactate
C3H5O3.Na 112.06 g/mol
|
CHEBI:232798
sodium L-lactate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source batch input: 1.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 14 | Yeast extract (BD-Difco) | Properties: Undefined component Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | ||
| 15 |
H2SO4
H2O4S 98.079 g/mol
|
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution Source pH-adjustment reagent with no fixed dose. Removed the unsupported VARIABLE placeholder. Original JCM213 specifies 1 N H2SO4 reagent; normality is not converted to molarity or a receiving-medium dose. | |
| 16 |
Carbon dioxide gas
CO2 44.009 g/mol
|
CHEBI:16526
carbon dioxide
|
Properties: ガス Source atmosphere context, not a fixed gas input or dissolved concentration. Removed the unsupported VARIABLE placeholder. | |
| 17 |
Nitrogen gas
N2 28.014 g/mol
|
CHEBI:17997
dinitrogen
|
Properties: ガス Source atmosphere context, not a fixed gas input or dissolved concentration. Removed the unsupported VARIABLE placeholder. |
Preparation
- Mix ingredients, except Na2S·9H2O, and adjust pH to 6.9 with 1 N H2SO4.
- Sterilize by filtration under a N2-CO2 (80:20) gas mixture.
- Neutralize Na2S·9H2O as a 5% solution and autoclave under a N2 atmosphere.
- Aseptically and anaerobically add the sterile Na2S·9H2O solution to the medium under a N2-CO2 (80:20) atmosphere, and readjust pH to 6.9, if necessary.
Solutions
Trace minerals (see Medium [M142])
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1 L | Source water batch input: 1 L = 1 L. This is not a measured final volume. |
| MgSO4・7H2O | Source batch input: 3 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| NaCl | Source batch input: 1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| CaCl2・2H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| Na2MoO4・2H2O | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| H3BO3 | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| FeSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| ZnSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| CuSO4・5H2O | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| CoSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| Nitrilotriacetic acid | Source batch input: 1.5 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| MnSO4・xH2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| AlK(SO4)2 | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. |
Cross-reference to Medium M142
Source stock addition: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter.
KOH solution
Stock pH-adjustment reagent with no fixed dose or numeric strength.
Na2S・9H2O separately prepared solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S・9H2O | Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. |
Separate sulfide preparation; composition notes retain the 0.5 g receiving-batch contribution, not a stock-batch recipe. Stock solvent volume and addition volume are unspecified. The source 5% label has unspecified percentage basis; no numeric stock strength or derived dose is asserted.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:54.611926Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M206 |
| 2026-01-28T19:48:24.200957Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:39.993780+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:59.118666Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008658 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.748054+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Archaeoglobus Fulgidus Medium" to "archaeoglobus_fulgidus_medium" for consistent matching |
| 2026-05-16T09:03:12.250343+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:35.407687+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.418789+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.575001+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T18:39:22.851782+00:00 | codex | RESTORED_FILTERED_MEDIA_STOCK_SCOPES_AND_BATCH_AMOUNTS_WITH_EVIDENCE | Restored batch inputs, referenced stock composition and separate sulfide preparation. Retained only source-explicit stock rates; removed unsupported final-rate and VARIABLE assertions with exact evidence. Preserved annotations and history; no final volume, percent basis or stock dose inferred. |