inorganic_salts_starch_agar_isp_4_with_0_05_yeast_extract (CultureMech:008702)

Overview

External ontology

TOGO:M210 — Inorganic Salts-Starch Agar (ISP-4) With 0.05% Yeast Extract

Applications

Microbial cultivation

pH range

{'min': 7.0, 'max': 7.4, 'notes': 'JCM Medium 58 says the unadjusted pH will be 7.0-7.4.'}

Notes

TOGO M210 mirrors JCM Medium 217: Inorganic Salts-Starch Agar (ISP-4) with 0.05% Yeast Extract supplements JCM Medium 58 ISP-4 with 0.5 g/L Yeast extract.

Composition

flowchart LR
    CultureMech_008702["inorganic_salts_starch_agar_isp_4_with_0_05_yeast_extract"]
    ing0_Soluble_starch["Soluble starch"]
    CultureMech_008702 --> ing0_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing0_Soluble_starch -.-> CHEBI_28017
    ing1_K2HPO4["K2HPO4"]
    CultureMech_008702 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008702 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaCl["NaCl"]
    CultureMech_008702 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008702 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_CaCO3["CaCO3"]
    CultureMech_008702 --> ing5_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing5_CaCO3 -.-> CHEBI_3311
    ing6_Agar["Agar"]
    CultureMech_008702 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Distilled_water["Distilled water"]
    CultureMech_008702 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_008702 --> ing8_Yeast_extract

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Soluble starch	10.0 G_PER_L	CHEBI:28017 starch	JCM Medium 58 prints this component for ISP-4.
2	K2HPO4 HO4P.2K 174.174 g/mol	1.0 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	JCM Medium 58 prints this component for ISP-4.
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	1.0 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	JCM Medium 58 prints this component for ISP-4.
4	NaCl Cl.Na 58.443 g/mol	1.0 G_PER_L	CHEBI:26710 sodium chloride	JCM Medium 58 prints this component for ISP-4.
5	(NH4)2SO4 2H4N.O4S 132.141 g/mol	2.0 G_PER_L	CHEBI:62946 ammonium sulfate	JCM Medium 58 prints this component for ISP-4.
6	CaCO3 CO3.Ca 100.086 g/mol	2.0 G_PER_L	CHEBI:3311 calcium carbonate	JCM Medium 58 prints this component for ISP-4.
7	Agar	20.0 G_PER_L	CHEBI:2509 agar	JCM Medium 58 prints this component for ISP-4.
8	Distilled water H2O 18.015 g/mol	1.0 L	CHEBI:15377 water	JCM Medium 58 prints this component for ISP-4.
9	Yeast extract	0.5 G_PER_L	FOODON:03315426 yeast extract	JCM Medium 217 supplements 1 L of JCM Medium 58 ISP-4 with 0.5 g Yeast extract.

Preparation

Mix the JCM Medium 58 ISP-4 ingredients with 1 mL/L Trace salts solution.
Autoclave at 121 C for 15 min.

Solutions

Trace salts solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
FeSO4 x 7 H2O	1 G_PER_L	JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
MnCl2 x 4 H2O	1 G_PER_L	JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.
ZnSO4 x 7 H2O	1 G_PER_L	JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.

JCM Medium 58 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.624945Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M210
2026-03-09T08:04:14.400505+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:19:59.535395Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008702
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T19:46:18.176156+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Inorganic Salts-Starch Agar (ISP-4) With 0.05% Yeast Extract" to "inorganic_salts_starch_agar_isp_4_with_0_05_yeast_extract" for consistent matching
2026-05-16T09:03:37.800433+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-09-06T00:00:00-07:00	repair_jcm_isp_supplements_score40.py	RESOLVED_JCM_ISP_SUPPLEMENTS_SCORE40_GRAPH	TOGO M210 mirrors JCM Medium 217: Inorganic Salts-Starch Agar (ISP-4) with 0.05% Yeast Extract supplements JCM Medium 58 ISP-4 with 0.5 g/L Yeast extract.