Overview
- External ontology
- TOGO:M2114 — AS-168 Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2114 Original source: NBRC - NBRC_M1439 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1439
Composition
flowchart LR
CultureMech_008707["as_168_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008707 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008707 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008707 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008707 --> ing3_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_7H2O -.-> CHEBI_75836
ing4_KCl["KCl"]
CultureMech_008707 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008707 --> ing5_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_4H2O -.-> CHEBI_86368
ing6_Sodium_glutamate["Sodium glutamate"]
CultureMech_008707 --> ing6_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing6_Sodium_glutamate -.-> CHEBI_64220
ing7_Trisodium_citrate["Trisodium citrate"]
CultureMech_008707 --> ing7_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing7_Trisodium_citrate -.-> CHEBI_53258
ing8_Agar__if_needed_["Agar (if needed)"]
CultureMech_008707 --> ing8_Agar__if_needed_
ing9_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008707 --> ing9_Bacto_Yeast_Extract__Difco_
ing10_Bacto_Casamino_acids__Difco_["Bacto Casamino acids (Difco)"]
CultureMech_008707 --> ing10_Bacto_Casamino_acids__Difco_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
150 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
50 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
1.8 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component |
| 8 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 9 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 10 | Bacto Yeast Extract (Difco) | 5 G_PER_L | Properties: Undefined component | |
| 11 | Bacto Casamino acids (Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.089771Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2114 |
| 2026-01-28T19:48:34.997837Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:59.604862Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008707 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.247025+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AS-168 Medium" to "as_168_medium" for consistent matching |
| 2026-06-05T07:40:49.830902+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:36.846972+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |