Overview
- External ontology
- TOGO:M2127 — 1/10 R2A with MES (pH6)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2127 Original source: NBRC - NBRC_M1459 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1459
Composition
flowchart LR
CultureMech_008720["1_10_r2a_with_mes_ph6"]
ing0_Distilled_water["Distilled water"]
CultureMech_008720 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MES["MES"]
CultureMech_008720 --> ing1_MES
CHEBI_39010["CHEBI:39010"]
ing1_MES -.-> CHEBI_39010
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008720 --> ing2_Agar__if_needed_
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | MES | 3.9 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 3 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component |
Solutions
R2A Broth \"DAIGO\"*
Dose: 0.32 G_PER_L
Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.131498Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2127 |
| 2026-01-28T19:48:26.648941Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:44.239150+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:59.798994Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008720 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.441215+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/10 R2A with MES (pH6)" to "1_10_r2a_with_mes_ph6" for consistent matching |
| 2026-05-16T09:02:58.027348+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:15.431419+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |