Overview
- External ontology
- TOGO:M212 — CYC Agar (pH 8.0)
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- TOGO M212/JCM Medium 219 prepares CYC Agar from TOGO M41/JCM Medium 49 and adjusts the complete medium to pH 8.0.
Composition
flowchart LR
CultureMech_008723["cyc_agar_ph_8_0"]
ing0_Czapek_Dox_liquid_medium__modified__Oxoid_["Czapek-Dox liquid medium, modified (Oxoid)"]
CultureMech_008723 --> ing0_Czapek_Dox_liquid_medium__modified__Oxoid_
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008723 --> ing1_Yeast_extract__BD_Difco_
ing2_Casamino_acids__vitamin_assay__BD_Difco_["Casamino acids, vitamin assay (BD-Difco)"]
CultureMech_008723 --> ing2_Casamino_acids__vitamin_assay__BD_Difco_
ing3_Agar["Agar"]
CultureMech_008723 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Distilled_water["Distilled water"]
CultureMech_008723 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Czapek-Dox liquid medium, modified (Oxoid) | 33.4 G_PER_L | TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. | |
| 2 | Yeast extract (BD-Difco) | 2 G_PER_L | TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. | |
| 3 | Casamino acids, vitamin assay (BD-Difco) | 6 G_PER_L | TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. | |
| 4 | Agar | 16 G_PER_L |
CHEBI:2509
agar
|
TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
TOGO M41 and JCM Medium 49 list this component for CYC Agar; TOGO M212 and JCM Medium 219 use the same formulation adjusted to pH 8.0. |
Preparation
- Dissolve the CYC Agar components in distilled water and bring the final volume to 1 L.
- Adjust the complete medium to pH 8.0.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.626367Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M212 |
| 2026-03-09T08:04:42.814196+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:59.813879Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008723 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:18.457303+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CYC Agar (pH 8.0)" to "cyc_agar_ph_8_0" for consistent matching |
| 2026-05-16T09:03:12.348393+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-06T00:00:00-07:00 | repair_jcm_ph_score40.py | RESOLVED_JCM_PH_SCORE40_GRAPH | TOGO M212/JCM Medium 219 prepares CYC Agar from TOGO M41/JCM Medium 49 and adjusts the complete medium to pH 8.0. |