bcye_agar

CultureMech:008727 bacterial COMPLEX SOLID_AGAR
Original name: BCYEα Agar

Overview

External ontology
TOGO:M2133 — BCYEα Agar
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M2133 imports NBRC Medium 1469 as BCYE alpha Agar. NBRC 1469 lists 10 g yeast extract, 0.25 g ferric pyrophosphate, 1.5 g activated charcoal, 10 g ACES buffer, 1 g alpha-Ketoglutarate, 0.4 g L-Cysteine, 1 L distilled water, 15 g agar, and pH 7.0. NBRC 1469 also notes microaerobic growth in an anaerobic jar equipped with a CO2 gas pack.

Composition

flowchart LR
    CultureMech_008727["bcye_agar"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_008727 --> ing0_Yeast_extract
    ing1_Ferric_pyrophosphate["Ferric pyrophosphate"]
    CultureMech_008727 --> ing1_Ferric_pyrophosphate
    CHEBI_132767["CHEBI:132767"]
    ing1_Ferric_pyrophosphate -.-> CHEBI_132767
    ing2_Charcoal_activated["Charcoal activated"]
    CultureMech_008727 --> ing2_Charcoal_activated
    ing3_ACES_buffer["ACES buffer"]
    CultureMech_008727 --> ing3_ACES_buffer
    CHEBI_39061["CHEBI:39061"]
    ing3_ACES_buffer -.-> CHEBI_39061
    ing4___Ketoglutarate["α-Ketoglutarate"]
    CultureMech_008727 --> ing4___Ketoglutarate
    ing5_L_Cysteine["L-Cysteine"]
    CultureMech_008727 --> ing5_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing5_L_Cysteine -.-> CHEBI_17561
    ing6_Distilled_water["Distilled water"]
    CultureMech_008727 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_Agar["Agar"]
    CultureMech_008727 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_CO2_gas["CO2 gas"]
    CultureMech_008727 --> ing8_CO2_gas
    CHEBI_16526["CHEBI:16526"]
    ing8_CO2_gas -.-> CHEBI_16526

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 10.0 G_PER_L FOODON:03315426
yeast extract
 NBRC Medium 1469 lists 10.0 g/L Yeast extract.
2 Ferric pyrophosphate
4Fe.3O7P2 745.203 g/mol
0.25 G_PER_L CHEBI:132767
ferric pyrophosphate
 NBRC Medium 1469 lists 0.25 g/L Ferric pyrophosphate.
3 Charcoal activated 1.5 G_PER_L NBRC Medium 1469 lists 1.5 g/L activated charcoal; this material is retained as an opaque protective component.
4 ACES buffer 10.0 G_PER_L CHEBI:39061
ACES
 NBRC Medium 1469 lists 10.0 g/L ACES buffer.
5 α-Ketoglutarate 1.0 G_PER_L NBRC Medium 1469 lists 1 g/L alpha-Ketoglutarate; this row is left ungrounded because the source does not specify the acid or salt form.
6 L-Cysteine
C3H7NO2S 121.161 g/mol
0.4 G_PER_L CHEBI:17561
L-cysteine
 NBRC Medium 1469 lists 0.4 g/L L-Cysteine.
7 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 NBRC Medium 1469 lists 1 L distilled water.
8 Agar 15.0 G_PER_L CHEBI:2509
agar
 NBRC Medium 1469 lists 15.0 g/L Agar.
9 CO2 gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 NBRC Medium 1469 notes microaerobic growth in an anaerobic jar equipped with a CO2 gas pack.

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:59.143979Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2133
2026-01-28T19:48:09.116865Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2 gas
2026-03-13T06:19:59.857146Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008727
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.501423+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BCYEα Agar" to "bcye_agar" for consistent matching
2026-05-16T09:03:12.354634+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:35.508116+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m2133_score15.py RESOLVED_TOGO_M2133_SCORE15 TOGO M2133 imports NBRC Medium 1469 as BCYE alpha Agar. NBRC 1469 lists 10 g yeast extract, 0.25 g ferric pyrophosphate, 1.5 g activated charcoal, 10 g ACES buffer, 1 g alpha-Ketoglutarate, 0.4 g L-Cysteine, 1 L distilled water, 15 g agar, and pH 7.0. NBRC 1469 also notes microaerobic growth in an anaerobic jar equipped with a CO2 gas pack. Corrected the imported water g/L artifact, added the NBRC pH, and marked the NBRC formula as curated.