Overview
- External ontology
- TOGO:M2143 — PYG medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2143 Original source: NBRC - NBRC_M1488 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1488
Composition
flowchart LR
CultureMech_008738["pyg_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_008738 --> ing0_Yeast_extract
ing1_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008738 --> ing1_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing1_Sodium_pyruvate -.-> CHEBI_50144
ing2_Glycerol["Glycerol"]
CultureMech_008738 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_Glycine_betaine["Glycine betaine"]
CultureMech_008738 --> ing3_Glycine_betaine
CHEBI_17750["CHEBI:17750"]
ing3_Glycine_betaine -.-> CHEBI_17750
ing4_Agar["Agar"]
CultureMech_008738 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Peptone["Peptone"]
CultureMech_008738 --> ing5_Peptone
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1.25 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
10 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 4 |
Glycine betaine
C5H11NO2 117.148 g/mol
|
1.25 G_PER_L |
CHEBI:17750
glycine betaine
|
Role: Growth factor; Properties: Defined component, Simple component, Organic compound |
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 | Peptone | 3 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.162579Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2143 |
| 2026-01-28T19:48:31.528873Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:59.942127Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008738 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.590836+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYG medium" to "pyg_medium" for consistent matching |
| 2026-06-05T07:40:35.528015+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.764242+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |