Overview
- External ontology
- TOGO:M2155 — R2A with ASW
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2155 Original source: NBRC - NBRC_M1510 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1510
Composition
flowchart LR
CultureMech_008749["r2a_with_asw"]
ing0_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008749 --> ing0_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_7H2O -.-> CHEBI_31795
ing1_K2HPO4["K2HPO4"]
CultureMech_008749 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008749 --> ing2_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Sodium_pyruvate -.-> CHEBI_50144
ing3_Glucose["Glucose"]
CultureMech_008749 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Soluble_starch["Soluble starch"]
CultureMech_008749 --> ing4_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing4_Soluble_starch -.-> CHEBI_28017
ing5_Agar__if_needed_["Agar (if needed)"]
CultureMech_008749 --> ing5_Agar__if_needed_
ing6_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008749 --> ing6_Bacto_Yeast_Extract__Difco_
ing7_Bacto_Proteose_Peptone_No__3__Difco_["Bacto Proteose Peptone No. 3 (Difco)"]
CultureMech_008749 --> ing7_Bacto_Proteose_Peptone_No__3__Difco_
ing8_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
CultureMech_008749 --> ing8_Bacto_Casamino_Acids__Difco_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 5 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 6 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 7 | Bacto Yeast Extract (Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 8 | Bacto Proteose Peptone No. 3 (Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 9 | Bacto Casamino Acids (Difco) | 0.5 G_PER_L | Properties: Undefined component |
Solutions
Seawater*
Dose: 1 G_PER_L
See mediadive_1801_Seawater.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.180192Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2155 |
| 2026-01-28T19:48:10.077270Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:16.405012+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:00.036923Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008749 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.688850+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A with ASW" to "r2a_with_asw" for consistent matching |
| 2026-05-16T09:04:30.227091+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:43.682634+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:30.747710+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.802397+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |