renibacterium_kdm_2_medium

CultureMech:008770 bacterial COMPLEX SOLID_AGAR
Original name: Renibacterium KDM-2 medium

Overview

External ontology
TOGO:M2176 — Renibacterium KDM-2 medium
Applications
  • Microbial cultivation
pH
6.5
Notes
TOGO M2176 imports ATCC Medium 1108 as Renibacterium KDM-2 medium. ATCC 1108 lists 1.0% w/v peptone, 0.05% w/v yeast extract, 0.1% w/v L-Cysteine . HCl, 1.5% w/v agar, and 20.0% fetal bovine serum. The ATCC preparation dissolves peptone, yeast extract, and cysteine in 80% final-volume distilled water, adjusts to pH 6.5 with NaOH, adds agar with heat, autoclaves for 15 minutes at 121 C, cools to 45 C, and adds serum at 45 C.

Composition

flowchart LR
    CultureMech_008770["renibacterium_kdm_2_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_008770 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008770 --> ing1_Yeast_extract
    ing2_L_Cysteine___HCl["L-Cysteine . HCl"]
    CultureMech_008770 --> ing2_L_Cysteine___HCl
    CHEBI_91247["CHEBI:91247"]
    ing2_L_Cysteine___HCl -.-> CHEBI_91247
    ing3_Agar["Agar"]
    CultureMech_008770 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_008770 --> ing4_Fetal_bovine_serum
    ing5_Distilled_water["Distilled water"]
    CultureMech_008770 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_NaOH["NaOH"]
    CultureMech_008770 --> ing6_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing6_NaOH -.-> CHEBI_32145

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Peptone 10.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 ATCC Medium 1108 lists 1.0% w/v peptone, normalized to 10 g/L.
2 Yeast extract 0.5 G_PER_L FOODON:03315426
yeast extract
 ATCC Medium 1108 lists 0.05% w/v yeast extract, normalized to 0.5 g/L.
3 L-Cysteine . HCl
C3H7NO2S.HCl 157.622 g/mol
1.0 G_PER_L CHEBI:91247
L-cysteine hydrochloride
 ATCC Medium 1108 lists 0.1% w/v L-Cysteine . HCl, normalized to 1 g/L.
4 Agar 15.0 G_PER_L CHEBI:2509
agar
 ATCC Medium 1108 lists 1.5% w/v agar, normalized to 15 g/L.
5 Fetal bovine serum 200.0 ML_PER_L ATCC Medium 1108 lists 20.0% fetal bovine serum added at 45 C, normalized to 200 ml/L; this serum is retained as an opaque complex component.
6 Distilled water
H2O 18.015 g/mol
800.0 ML_PER_L CHEBI:15377
water
 ATCC Medium 1108 dissolves peptone, yeast extract, and L-Cysteine . HCl in 80% final-volume distilled water, normalized to 800 ml/L.
7 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 ATCC Medium 1108 adjusts to pH 6.5 with NaOH but does not state a final NaOH amount.

Preparation

  1. Dissolve peptone, yeast extract, and L-Cysteine . HCl in distilled water.
  2. Adjust to pH 6.5 with NaOH.
  3. Add agar and dissolve by heating.
  4. Autoclave at 121 C for 15 minutes.
  5. Cool to 45 C.
  6. Add fetal bovine serum at 45 C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.124549Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2176
2026-01-28T19:48:31.443092Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-13T06:20:00.186474Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008770
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.850050+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Renibacterium KDM-2 medium" to "renibacterium_kdm_2_medium" for consistent matching
2026-05-16T09:03:12.471505+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:35.625565+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m2176_score15.py RESOLVED_TOGO_M2176_SCORE15 TOGO M2176 imports ATCC Medium 1108 as Renibacterium KDM-2 medium. ATCC 1108 lists 1.0% w/v peptone, 0.05% w/v yeast extract, 0.1% w/v L-Cysteine . HCl, 1.5% w/v agar, and 20.0% fetal bovine serum. The ATCC preparation dissolves peptone, yeast extract, and cysteine in 80% final-volume distilled water, adjusts to pH 6.5 with NaOH, adds agar with heat, autoclaves for 15 minutes at 121 C, cools to 45 C, and adds serum at 45 C. Corrected the imported percent/water units, added the ATCC pH and autoclave steps, and marked the ATCC formula as curated.