jxt_medium

CultureMech:008774 bacterial COMPLEX LIQUID
Original name: JXT Medium

Overview

External ontology
TOGO:M217 — JXT Medium
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
TOGO M217 imports JCM Medium 224 as JXT Medium. JCM 224 lists 1 g Yeast extract from BD-Difco, 1 g Trypticase peptone from BD-BBL, 1 g Na2S2O3·5H2O, and 1 L seawater at pH 7.0-7.2. The source says to use filtrated natural seawater or artificial seawater such as Jamarin S from Jamarin Laboratory, and to autoclave media at 121 C for 15 min unless otherwise stated.

Composition

flowchart LR
    CultureMech_008774["jxt_medium"]
    ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_008774 --> ing0_Yeast_extract__BD_Difco_
    ing1_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_008774 --> ing1_Trypticase_peptone__BD_BBL_
    ing2_Na2S2O3_5H2O["Na2S2O3·5H2O"]
    CultureMech_008774 --> ing2_Na2S2O3_5H2O
    CHEBI_32150["CHEBI:32150"]
    ing2_Na2S2O3_5H2O -.-> CHEBI_32150
    ing3_Seawater["Seawater"]
    CultureMech_008774 --> ing3_Seawater

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Yeast extract (BD-Difco) 1.0 G_PER_L FOODON:03315426
yeast extract
JCM Medium 224 lists 1 g/L Yeast extract from BD-Difco.
2 Trypticase peptone (BD-BBL) 1.0 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
JCM Medium 224 lists 1 g/L Trypticase peptone from BD-BBL.
3 Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
1.0 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
JCM Medium 224 lists 1.0 g/L Na2S2O3·5H2O.
4 Seawater 1.0 L JCM Medium 224 lists 1 L seawater and allows filtrated natural seawater or artificial seawater such as Jamarin S from Jamarin Laboratory; the seawater input is retained as an opaque complex component.

Preparation

  1. Adjust pH to 7.0-7.2.
  2. Autoclave at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.635835Z togo-import Imported from TOGO Medium Source: JCM, ID: M217
2026-03-13T06:20:00.210706Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008774
2026-03-17T19:46:18.875606+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JXT Medium" to "jxt_medium" for consistent matching
2026-09-11T00:00:00-07:00 repair_togo_m217_score15.py RESOLVED_TOGO_M217_SCORE15 TOGO M217 imports JCM Medium 224 as JXT Medium. JCM 224 lists 1 g Yeast extract from BD-Difco, 1 g Trypticase peptone from BD-BBL, 1 g Na2S2O3·5H2O, and 1 L seawater at pH 7.0-7.2. The source says to use filtrated natural seawater or artificial seawater such as Jamarin S from Jamarin Laboratory, and to autoclave media at 121 C for 15 min unless otherwise stated. Corrected the seawater unit, added the JCM pH range and default autoclave step, and marked the JCM formula as curated.