Overview
- External ontology
- TOGO:M217 — JXT Medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- TOGO M217 imports JCM Medium 224 as JXT Medium. JCM 224 lists 1 g Yeast extract from BD-Difco, 1 g Trypticase peptone from BD-BBL, 1 g Na2S2O3·5H2O, and 1 L seawater at pH 7.0-7.2. The source says to use filtrated natural seawater or artificial seawater such as Jamarin S from Jamarin Laboratory, and to autoclave media at 121 C for 15 min unless otherwise stated.
Composition
flowchart LR
CultureMech_008774["jxt_medium"]
ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008774 --> ing0_Yeast_extract__BD_Difco_
ing1_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_008774 --> ing1_Trypticase_peptone__BD_BBL_
ing2_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_008774 --> ing2_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing2_Na2S2O3_5H2O -.-> CHEBI_32150
ing3_Seawater["Seawater"]
CultureMech_008774 --> ing3_Seawater
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract (BD-Difco) | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
JCM Medium 224 lists 1 g/L Yeast extract from BD-Difco. |
| 2 | Trypticase peptone (BD-BBL) | 1.0 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
JCM Medium 224 lists 1 g/L Trypticase peptone from BD-BBL. |
| 3 |
Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.0 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
JCM Medium 224 lists 1.0 g/L Na2S2O3·5H2O. |
| 4 | Seawater | 1.0 L | JCM Medium 224 lists 1 L seawater and allows filtrated natural seawater or artificial seawater such as Jamarin S from Jamarin Laboratory; the seawater input is retained as an opaque complex component. |
Preparation
- Adjust pH to 7.0-7.2.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.635835Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M217 |
| 2026-03-13T06:20:00.210706Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008774 |
| 2026-03-17T19:46:18.875606+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JXT Medium" to "jxt_medium" for consistent matching |
| 2026-09-11T00:00:00-07:00 | repair_togo_m217_score15.py | RESOLVED_TOGO_M217_SCORE15 | TOGO M217 imports JCM Medium 224 as JXT Medium. JCM 224 lists 1 g Yeast extract from BD-Difco, 1 g Trypticase peptone from BD-BBL, 1 g Na2S2O3·5H2O, and 1 L seawater at pH 7.0-7.2. The source says to use filtrated natural seawater or artificial seawater such as Jamarin S from Jamarin Laboratory, and to autoclave media at 121 C for 15 min unless otherwise stated. Corrected the seawater unit, added the JCM pH range and default autoclave step, and marked the JCM formula as curated. |