anoxic_bicarbonate_buffered_basal_medium_bbm_with_10_mm_sodium_nitrate_and_various_concentrations_of_sodium_acetate (CultureMech:008779)

Overview

External ontology

TOGO:M2185 — Anoxic bicarbonate-buffered basal medium (BBM) with 10 mM sodium nitrate and various concentrations of sodium acetate

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2185

Composition

flowchart LR
    CultureMech_008779["anoxic_bicarbonate_buffered_basal_medium_bbm_with_10_mm_sodium_nitr..."]
    ing0_Sodium_acetate["Sodium acetate"]
    CultureMech_008779 --> ing0_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing0_Sodium_acetate -.-> CHEBI_32954
    ing1_Sodium_nitrate["Sodium nitrate"]
    CultureMech_008779 --> ing1_Sodium_nitrate
    CHEBI_63005["CHEBI:63005"]
    ing1_Sodium_nitrate -.-> CHEBI_63005
    ing2_Bicarbonate_buffered_basal_medium__BBM_["Bicarbonate-buffered basal medium (BBM)"]
    CultureMech_008779 --> ing2_Bicarbonate_buffered_basal_medium__BBM_
    ing3_Distilled_water["Distilled water"]
    CultureMech_008779 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_NH4Cl["NH4Cl"]
    CultureMech_008779 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KCl["KCl"]
    CultureMech_008779 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaHCO3["NaHCO3"]
    CultureMech_008779 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_NaH2PO4["NaH2PO4"]
    CultureMech_008779 --> ing7_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing7_NaH2PO4 -.-> CHEBI_37585
    ing8_Vitamins["Vitamins"]
    CultureMech_008779 --> ing8_Vitamins
    ing9_Minerals["Minerals"]
    CultureMech_008779 --> ing9_Minerals
    ing10_Biotin["Biotin"]
    CultureMech_008779 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_008779 --> ing11_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing12_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_008779 --> ing12_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_HCl -.-> CHEBI_30961
    ing13_Folic_acid["Folic acid"]
    CultureMech_008779 --> ing13_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing13_Folic_acid -.-> CHEBI_27470
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_008779 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843
    ing15_Riboflavin["Riboflavin"]
    CultureMech_008779 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_008779 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_Thiamine["Thiamine"]
    CultureMech_008779 --> ing17_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing17_Thiamine -.-> CHEBI_18385
    ing18_Pantothenic_acid["Pantothenic acid"]
    CultureMech_008779 --> ing18_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing18_Pantothenic_acid -.-> CHEBI_7916
    ing19_Thioctic_acid["Thioctic acid"]
    CultureMech_008779 --> ing19_Thioctic_acid
    CHEBI_30314["CHEBI:30314"]
    ing19_Thioctic_acid -.-> CHEBI_30314
    ing20_NaCl["NaCl"]
    CultureMech_008779 --> ing20_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing20_NaCl -.-> CHEBI_26710
    ing21_CaCl2_2H2O["CaCl2.2H2O"]
    CultureMech_008779 --> ing21_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing21_CaCl2_2H2O -.-> CHEBI_86158
    ing22_H3BO3["H3BO3"]
    CultureMech_008779 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_FeSO4_7H2O["FeSO4.7H2O"]
    CultureMech_008779 --> ing23_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing23_FeSO4_7H2O -.-> CHEBI_75836
    ing24_CoCl2_6H2O["CoCl2.6H2O"]
    CultureMech_008779 --> ing24_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing24_CoCl2_6H2O -.-> CHEBI_35696
    ing25_NiCl2_6H2O["NiCl2.6H2O"]
    CultureMech_008779 --> ing25_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_6H2O -.-> CHEBI_34887
    ing26_ZnCl2["ZnCl2"]
    CultureMech_008779 --> ing26_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing26_ZnCl2 -.-> CHEBI_49976
    ing27_AlK_SO4_2_12H2O["AlK(SO4)2.12H2O"]
    CultureMech_008779 --> ing27_AlK_SO4_2_12H2O
    CHEBI_86465["CHEBI:86465"]
    ing27_AlK_SO4_2_12H2O -.-> CHEBI_86465
    ing28_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_008779 --> ing28_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing28_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing29_Na2WO4_2H2O["Na2WO4.2H2O"]
    CultureMech_008779 --> ing29_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing29_Na2WO4_2H2O -.-> CHEBI_63939
    CultureMech_008779 --> more["...4 more"]

Ingredients (34)

#	Ingredient	Concentration	Ontology	Notes
1	Sodium acetate C2H3O2.Na 82.034 g/mol	6.25 MILLIMOLAR	CHEBI:32954 sodium acetate	Role: Buffer; Properties: Organic compound, Defined component, Simple component
2	Sodium nitrate NO3.Na 84.994 g/mol	10 MILLIMOLAR	CHEBI:63005 sodium nitrate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
3	Bicarbonate-buffered basal medium (BBM)	1 G_PER_L
4	Distilled water H2O 18.015 g/mol	1.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0]
5	NH4Cl Cl.H4N 53.492 g/mol	0.25 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6	KCl Cl.K 74.551 g/mol	0.1 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7	NaHCO3 CHO3.Na 84.006 g/mol	2.52 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
8	NaH2PO4 H2O4P.Na 119.976 g/mol	0.6 G_PER_L	CHEBI:37585 sodium dihydrogenphosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9	Vitamins	10 G_PER_L
10	Minerals	10 G_PER_L
11	Biotin C10H16N2O3S 244.316 g/mol	2 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	5 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13	Pyridoxine HCl C8H11NO3.HCl 205.641 g/mol	10 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14	Folic acid C19H19N7O6 441.404 g/mol	2 G_PER_L	CHEBI:27470 folic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15	Vitamin B12	0.1 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16	Riboflavin C17H20N4O6 376.369 g/mol	5 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
17	Nicotinic acid C6H5NO2 123.111 g/mol	5 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18	Thiamine C12H17N4OS 265.362 g/mol	5 G_PER_L	CHEBI:18385 thiamine(1+)	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
19	Pantothenic acid C9H17NO5 219.237 g/mol	5 G_PER_L	CHEBI:7916 pantothenic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
20	Thioctic acid C8H14O2S2 206.332 g/mol	5 G_PER_L	CHEBI:30314 (R)-lipoic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
21	NaCl Cl.Na 58.443 g/mol	1 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22	CaCl2.2H2O Ca.2Cl.2H2O 147.014 g/mol	0.1 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23	H3BO3 H3BO3 61.833 g/mol	0.01 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24	FeSO4.7H2O Fe.7H2O.O4S 278.013 g/mol	0.1 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25	CoCl2.6H2O 2Cl.Co 129.839 g/mol	0.1 G_PER_L	CHEBI:35696 cobalt dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26	NiCl2.6H2O Cl2Ni 129.599 g/mol	0.024 G_PER_L	CHEBI:34887 nickel dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27	ZnCl2 2Cl.Zn 136.296 g/mol	0.13 G_PER_L	CHEBI:49976 zinc dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
28	AlK(SO4)2.12H2O Al.12H2O.K.2O4S 474.386 g/mol	0.01 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
29	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	1.5 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Role: Mineral source; Properties: Organic compound, Defined component, Simple component
30	Na2WO4.2H2O 2H2O.2Na.O4W 329.846 g/mol	0.025 G_PER_L	CHEBI:63939 sodium tungstate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
31	MnSO4.H2O H2O.Mn.O4S 169.016 g/mol	0.5 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
32	MgSO4 Mg.O4S 120.368 g/mol	3 G_PER_L	CHEBI:32599 magnesium sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
33	CuSO4 Cu.O4S 159.609 g/mol	0.01 G_PER_L	CHEBI:23414 copper(II) sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
34	Na2MoO4 MoO4.2Na 205.916 g/mol	0.025 G_PER_L	CHEBI:75215 sodium molybdate (anhydrous)	Role: Mineral source; Properties: Organic compound, Defined component, Simple component

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.139206Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2185
2026-01-28T19:48:22.406853Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:07.076198Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 2 duplicate ingredient(s)
2026-03-13T06:20:00.258279Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008779
2026-03-17T06:30:13.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.924741+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Anoxic bicarbonate-buffered basal medium (BBM) with 10 mM sodium nitrate and various concentrations of sodium acetate" to "anoxic_bicarbonate_buffered_basal_medium_bbm_with_10_mm_sodium_nitrate_and_various_concentrations_of_sodium_acetate" for consistent matching
2026-06-05T07:40:49.233661+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:35.691305+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24)	Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:53.359293+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:56.618260+00:00	repair_merged_duplicates.py	REPAIRED_SUMMED_DUPLICATE_MERGE	Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.