hib_medium_columbia_agar_supplemented_with_5_g_l_yeast_extract_15_g_ml_nad_and_15_g_ml_haemin

CultureMech:008781 bacterial COMPLEX SOLID_AGAR
Original name: Hib medium (Columbia agar supplemented with 5 g/l yeast extract, 15 μg/ml NAD and 15 μg/ml haemin)

Overview

External ontology
TOGO:M2189 — Hib medium (Columbia agar supplemented with 5 g/l yeast extract, 15 μg/ml NAD and 15 μg/ml haemin)
Applications
  • Microbial cultivation
Notes
TOGO M2189 Hib medium supplements 1.0 L Columbia agar with 5.0 g/L yeast extract, 15.0 mg/L NAD, and 15.0 mg/L haemin. The source does not disclose the Columbia agar base formulation.

Composition

flowchart LR
    CultureMech_008781["hib_medium_columbia_agar_supplemented_with_5_g_l_yeast_extract_15_g..."]
    ing0_Columbia_agar["Columbia agar"]
    CultureMech_008781 --> ing0_Columbia_agar
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008781 --> ing1_Yeast_extract
    ing2_NAD["NAD"]
    CultureMech_008781 --> ing2_NAD
    CHEBI_15846["CHEBI:15846"]
    ing2_NAD -.-> CHEBI_15846
    ing3_Haemin["Haemin"]
    CultureMech_008781 --> ing3_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing3_Haemin -.-> CHEBI_50385

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Columbia agar 1000.0 ML_PER_L TOGO M2189 lists 1.0 L Columbia agar; this commercial agar base is retained as an opaque complex component.
2 Yeast extract 5.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M2189 lists 5.0 g/L Yeast extract.
3 NAD
C21H28N7O14P2 664.438 g/mol
15.0 MG_PER_L CHEBI:15846
NAD(+)
 TOGO M2189 lists 15 ug/ml NAD, equivalent to 15.0 mg/L.
4 Haemin
C34H32ClFeN4O4 651.952 g/mol
15.0 MG_PER_L CHEBI:50385
hemin
 TOGO M2189 lists 15 ug/ml haemin, equivalent to 15.0 mg/L.

Preparation

  1. Supplement 1.0 L Columbia agar with 5.0 g/L yeast extract, 15.0 mg/L NAD, and 15.0 mg/L haemin.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.146631Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2189
2026-01-28T19:48:21.654293Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.279415Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008781
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.946042+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Hib medium (Columbia agar supplemented with 5 g/l yeast extract, 15 μg/ml NAD and 15 μg/ml haemin)" to "hib_medium_columbia_agar_supplemented_with_5_g_l_yeast_extract_15_g_ml_nad_and_15_g_ml_haemin" for consistent matching
2026-06-05T07:41:08.495989+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2189_hib_score15.py RESOLVED_TOGO_M2189_HIB_SCORE15 Converted 15 ug/ml NAD and haemin to 15.0 mg/L, represented Columbia agar as an opaque 1.0 L commercial base, grounded the disclosed yeast extract/NAD/haemin components, and retained Columbia agar as an intentionally unmapped component.