nutrient_agar_broth

CultureMech:008785 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient Agar/Broth

Overview

External ontology
TOGO:M2192 — Nutrient Agar/Broth
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2192 Original URL: https://www.atcc.org/~/media/E6FA2163B72E4DCD880719A2612F2C92.ashx Commercial Product: Nutrient Agar Supplier: Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Catalog: TM Media, BD 213000 Source: https://microbenotes.com/nutrient-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_008785["nutrient_agar_broth"]
    ing0_DI_Water["DI Water"]
    CultureMech_008785 --> ing0_DI_Water
    ing1_Peptone["Peptone"]
    CultureMech_008785 --> ing1_Peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008785 --> ing2_Yeast_extract
    ing3_Beef_extract["Beef extract"]
    CultureMech_008785 --> ing3_Beef_extract
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_008785 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Agar["Agar"]
    CultureMech_008785 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Agar["Agar"]
    CultureMech_008785 --> ing6_Agar
    ing6_Agar -.-> CHEBI_2509
    ing7_Beef_Extract["Beef Extract"]
    CultureMech_008785 --> ing7_Beef_Extract
    ing8_Peptone["Peptone"]
    CultureMech_008785 --> ing8_Peptone

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 DI Water 1000 G_PER_L Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Peptone
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
5.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
Enzymatic digest of protein, provides nitrogen, amino acids, and peptides; Constituent of Nutrient Agar commercial product
3 Yeast extract
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
1.5 G_PER_L FOODON:03315426
yeast extract
Provides B-complex vitamins and additional nutrients; Constituent of Nutrient Agar commercial product
4 Beef extract
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
1.5 G_PER_L FOODON:03302088
beef extract
Aqueous extract of beef tissue, provides organic compounds and minerals; Constituent of Nutrient Agar commercial product
5 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
5.0 G_PER_L CHEBI:26710
sodium chloride
Maintains osmotic equilibrium; Constituent of Nutrient Agar commercial product
6 Agar
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
15.0 G_PER_L CHEBI:2509
agar
Solidifying agent. Omit for Nutrient Broth (liquid variant); Constituent of Nutrient Agar commercial product
7 Agar 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component
8 Beef Extract 3 G_PER_L Properties: Undefined component
9 Peptone 5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.149335Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2192
2026-01-28T19:48:23.191750Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.293999Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008785
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.961664+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient Agar/Broth" to "nutrient_agar_broth" for consistent matching
2026-06-05T07:40:35.685987+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.