nutrient_agar_broth

CultureMech:008786 bacterial COMPLEX LIQUID
Original name: Nutrient Agar/Broth

Overview

External ontology
TOGO:M2193 — Nutrient Agar/Broth
Applications
  • Microbial cultivation
pH
6.8
Notes
TOGO M2193 cites ATCC Medium 3; ATCC Medium 3 lists 8.0 g Nutrient Broth per 1000 mL DI water and prints a scratch broth formulation with agar omitted.

Composition

flowchart LR
    CultureMech_008786["nutrient_agar_broth"]
    ing0_Beef_Extract["Beef Extract"]
    CultureMech_008786 --> ing0_Beef_Extract
    ing1_Peptone["Peptone"]
    CultureMech_008786 --> ing1_Peptone
    ing2_DI_Water["DI Water"]
    CultureMech_008786 --> ing2_DI_Water
    CHEBI_15377["CHEBI:15377"]
    ing2_DI_Water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Beef Extract 3.0 G_PER_L FOODON:03302088
beef extract
ATCC Medium 3 lists 3.0 g beef extract in the Nutrient Agar scratch recipe and omits agar for Nutrient Broth.
2 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
ATCC Medium 3 lists 5.0 g peptone in the Nutrient Agar scratch recipe and omits agar for Nutrient Broth.
3 DI Water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
ATCC Medium 3 lists 1000 mL deionized water.

Preparation

  1. Prepare Nutrient Broth with 3.0 g beef extract and 5.0 g peptone in 1000 mL DI water.
  2. Autoclave at 121 C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.150220Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2193
2026-03-13T06:20:00.296720Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008786
2026-03-17T19:46:18.964464+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient Agar/Broth" to "nutrient_agar_broth" for consistent matching
2026-09-07T00:00:00-07:00 repair_togo_exact_score25.py RESOLVED_TOGO_EXACT_SCORE25_GRAPH TOGO M2193 cites ATCC Medium 3; ATCC Medium 3 lists 8.0 g Nutrient Broth per 1000 mL DI water and prints a scratch broth formulation with agar omitted.