nutrient_broth_nb_difco

CultureMech:008787 bacterial COMPLEX LIQUID
Original name: Nutrient broth (NB) (Difco)

Overview

External ontology
TOGO:M2194 — Nutrient broth (NB) (Difco)
Applications
  • Microbial cultivation
pH range
{'min': 6.6, 'max': 7.0}
Notes
TOGO M2194 lists 8 g Nutrient broth (NB) from Difco in one liter of distilled water at pH 6.8 +/- 0.2.

Composition

flowchart LR
    CultureMech_008787["nutrient_broth_nb_difco"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008787 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Nutrient_broth__NB___Difco_["Nutrient broth (NB) (Difco)"]
    CultureMech_008787 --> ing1_Nutrient_broth__NB___Difco_

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
TOGO M2194 lists one liter of distilled water.
2 Nutrient broth (NB) (Difco) 8 G_PER_L TOGO M2194 lists 8 G_PER_L of Nutrient broth (NB) (Difco).

Preparation

  1. Dissolve 8 g Nutrient broth (NB) (Difco) in one liter of distilled water.
  2. Cultivate at 30 C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.151019Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2194
2026-01-28T19:48:41.652867Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.299196Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008787
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.967138+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient broth (NB) (Difco)" to "nutrient_broth_nb_difco" for consistent matching
2026-06-05T07:41:40.176665+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_togo_condition_wrappers_score20.py RESOLVED_TOGO_CONDITION_WRAPPERS_SCORE20 TOGO M2194 lists 8 g Nutrient broth (NB) from Difco in one liter of distilled water at pH 6.8 +/- 0.2.