Overview
- External ontology
- TOGO:M2195 — King's B medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2195
Composition
flowchart LR
CultureMech_008788["kings_b_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008788 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Magnesium_sulphate_heptahydrate["Magnesium sulphate heptahydrate"]
CultureMech_008788 --> ing1_Magnesium_sulphate_heptahydrate
CHEBI_31795["CHEBI:31795"]
ing1_Magnesium_sulphate_heptahydrate -.-> CHEBI_31795
ing2_Dipotassium_hydrogen_phosphate["Dipotassium hydrogen phosphate"]
CultureMech_008788 --> ing2_Dipotassium_hydrogen_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Dipotassium_hydrogen_phosphate -.-> CHEBI_131527
ing3_glycerol["glycerol"]
CultureMech_008788 --> ing3_glycerol
CHEBI_17754["CHEBI:17754"]
ing3_glycerol -.-> CHEBI_17754
ing4_Proteose_peptone_["Proteose peptone "]
CultureMech_008788 --> ing4_Proteose_peptone_
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component |
| 2 |
Magnesium sulphate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
|
1.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
Dipotassium hydrogen phosphate
HO4P.2K 174.174 g/mol
|
1.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
glycerol
C3H8O3 92.094 g/mol
|
15 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 5 | Proteose peptone | 20 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.151720Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2195 |
| 2026-01-28T19:48:42.681482Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.302428Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008788 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.970833+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "King's B medium" to "kings_b_medium" for consistent matching |
| 2026-06-05T07:41:10.368918+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.259202+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |