Overview
- External ontology
- TOGO:M2198 — LB Agar/Broth, Miller
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2198 Original URL: https://www.atcc.org/~/media/6F57EBDFF28049EE85F7C697DAFB2592.ashx Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008790["lb_agar_broth_miller"]
ing0_DI_Water["DI Water"]
CultureMech_008790 --> ing0_DI_Water
ing1_Tryptone["Tryptone"]
CultureMech_008790 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_008790 --> ing2_Yeast_extract
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_008790 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Yeast_Extract["Yeast Extract"]
CultureMech_008790 --> ing4_Yeast_Extract
ing5_Sodium_Chloride["Sodium Chloride"]
CultureMech_008790 --> ing5_Sodium_Chloride
ing5_Sodium_Chloride -.-> CHEBI_26710
ing6_Agar["Agar"]
CultureMech_008790 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Tryptone["Tryptone"]
CultureMech_008790 --> ing7_Tryptone
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DI Water | 1000 G_PER_L | Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound | |
| 2 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 3 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 | Yeast Extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
Sodium Chloride
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Tryptone | 10 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.153787Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2198 |
| 2026-01-28T19:48:18.667218Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.311173Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008790 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.980491+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB Agar/Broth, Miller" to "lb_agar_broth_miller" for consistent matching |
| 2026-06-05T07:41:10.799084+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |