medium_1_liquid_with_20_horse_serum

CultureMech:008794 bacterial COMPLEX SOLID_AGAR
Original name: Medium 1 liquid, with 20 % horse serum

Overview

External ontology
TOGO:M2200 — Medium 1 liquid, with 20 % horse serum
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M2200 imports DSMZ Medium 1 with a 20% horse-serum supplement. The TOGO M2200 component table lists 5 g Peptone, 3 g Meat extract, 20% Horse serum, 15 g Agar, and 1000 ml Distilled water; the source pH is 7.0. TOGO M2200 retains the DSMZ Medium 1 agar row despite the medium name containing liquid.

Composition

flowchart LR
    CultureMech_008794["medium_1_liquid_with_20_horse_serum"]
    ing0_Peptone["Peptone"]
    CultureMech_008794 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_008794 --> ing1_Meat_extract
    ing2_Horse_serum["Horse serum"]
    CultureMech_008794 --> ing2_Horse_serum
    ing3_Agar["Agar"]
    CultureMech_008794 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_008794 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
DSMZ Medium 1 and TOGO M2200 list 5 g/L Peptone.
2 Meat extract 3.0 G_PER_L DSMZ Medium 1 and TOGO M2200 list 3 g/L Meat extract; this generic complex extract is retained without an ontology grounding.
3 Horse serum 20.0 PERCENT_V_V TOGO M2200 lists the horse-serum supplement as 20%; this liquid serum addition is represented as % v/v and retained without an ontology grounding.
4 Agar 15.0 G_PER_L CHEBI:2509
agar
TOGO M2200 carries the DSMZ Medium 1 15 g/L Agar row.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
DSMZ Medium 1 and TOGO M2200 list 1000 ml Distilled water.

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.155726Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2200
2026-01-28T19:48:31.215794Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.328339Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008794
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.998353+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium 1 liquid, with 20 % horse serum" to "medium_1_liquid_with_20_horse_serum" for consistent matching
2026-06-05T07:41:15.884354+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2200_horse_serum_score15.py RESOLVED_TOGO_M2200_SCORE15 TOGO M2200 imports DSMZ Medium 1 with a 20% horse-serum supplement. The TOGO M2200 component table lists 5 g Peptone, 3 g Meat extract, 20% Horse serum, 15 g Agar, and 1000 ml Distilled water; the source pH is 7.0. TOGO M2200 retains the DSMZ Medium 1 agar row despite the medium name containing liquid. Corrected the imported water unit and horse-serum percent artifacts, grounded the disclosed simple and protein-hydrolysate components, kept Meat extract and Horse serum intentionally unmapped, and linked the record to DSMZ Medium 1.