Overview
- External ontology
- TOGO:M2222 — 2.5% NaCl heart infusion (HI) medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2222
Composition
flowchart LR
CultureMech_008810["2_5_nacl_heart_infusion_hi_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008810 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008810 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Chloramphenicol["Chloramphenicol"]
CultureMech_008810 --> ing2_Chloramphenicol
CHEBI_17698["CHEBI:17698"]
ing2_Chloramphenicol -.-> CHEBI_17698
ing3_Ampicillin["Ampicillin"]
CultureMech_008810 --> ing3_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing3_Ampicillin -.-> CHEBI_28971
ing4_Tetracycline["Tetracycline"]
CultureMech_008810 --> ing4_Tetracycline
CHEBI_27902["CHEBI:27902"]
ing4_Tetracycline -.-> CHEBI_27902
ing5_Heart_infusion_broth["Heart infusion broth"]
CultureMech_008810 --> ing5_Heart_infusion_broth
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
2.5 PERCENT_W_V |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
Chloramphenicol
C11H12Cl2N2O5 323.132 g/mol
|
2 G_PER_L |
CHEBI:17698
chloramphenicol
|
Role: Antimicrobial agent; Properties: Organic compound, Simple component, Defined component |
| 4 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
20 G_PER_L |
CHEBI:28971
ampicillin
|
Role: Antimicrobial agent; Properties: Simple component, Defined component, Organic compound |
| 5 |
Tetracycline
C22H24N2O8 444.44 g/mol
|
2 G_PER_L |
CHEBI:27902
tetracycline
|
Role: Antimicrobial agent; Properties: Simple component, Defined component |
| 6 | Heart infusion broth | 37 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.174757Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2222 |
| 2026-01-28T19:48:42.369321Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.431437Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008810 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.105553+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "2.5% NaCl heart infusion (HI) medium" to "2_5_nacl_heart_infusion_hi_medium" for consistent matching |
| 2026-06-05T07:40:15.737394+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-20T19:01:00.170811+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 2 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |