Overview
- External ontology
- TOGO:M2225 — De Man-Rogosa-Sharpe (MRS) broth (Biokar Diagnostic)
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.2, 'max': 6.6}
- Notes
- TOGO M2225 lists 55.3 g De Man-Rogosa-Sharpe broth from Biokar Diagnostic in one liter of distilled water at pH 6.4 +/- 0.2.
Composition
flowchart LR
CultureMech_008813["de_man_rogosa_sharpe_mrs_broth_biokar_diagnostic"]
ing0_Distilled_water["Distilled water"]
CultureMech_008813 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_De_Man_Rogosa_Sharpe__MRS__broth__Biokar_Diagnostic_["De Man-Rogosa-Sharpe (MRS) broth (Biokar Diagnostic)"]
CultureMech_008813 --> ing1_De_Man_Rogosa_Sharpe__MRS__broth__Biokar_Diagnostic_
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
TOGO M2225 lists one liter of distilled water. |
| 2 | De Man-Rogosa-Sharpe (MRS) broth (Biokar Diagnostic) | 55.3 G_PER_L | TOGO M2225 lists 55.3 G_PER_L of De Man-Rogosa-Sharpe (MRS) broth (Biokar Diagnostic). |
Preparation
- Dissolve 55.3 g De Man-Rogosa-Sharpe broth in one liter of distilled water.
- Cultivate anaerobically for 24 h at 37 C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.178076Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2225 |
| 2026-01-28T19:48:10.604369Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.443962Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008813 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.118573+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "De Man-Rogosa-Sharpe (MRS) broth (Biokar Diagnostic)" to "de_man_rogosa_sharpe_mrs_broth_biokar_diagnostic" for consistent matching |
| 2026-06-05T07:40:55.351316+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_togo_condition_wrappers_score20.py | RESOLVED_TOGO_CONDITION_WRAPPERS_SCORE20 | TOGO M2225 lists 55.3 g De Man-Rogosa-Sharpe broth from Biokar Diagnostic in one liter of distilled water at pH 6.4 +/- 0.2. |