mycoplasma_medium_atcc_243_with_sucrose

Overview

External ontology

TOGO:M2227 — Mycoplasma medium (ATCC 243) with sucrose

Applications

• Microbial cultivation

pH range

{'min': 7.2, 'max': 7.6}

Notes

TOGO M2227 imports ATCC Medium 1161, Mycoplasma medium (ATCC 243) with sucrose. ATCC 1161 prepares the basal agar from 17.5 g Heart Infusion Broth, 10.0 g Agar Noble, and 450.0 ml water; separately filter-sterilizes 40.0 g Sucrose in 200.0 ml water; and aseptically combines the cooled basal agar with the sterile sucrose, 150.0 ml 15% Yeast extract solution, and 200.0 ml heat-inactivated Horse serum.

Composition

flowchart LR
    CultureMech_008815["mycoplasma_medium_atcc_243_with_sucrose"]
    ing0_Distilled_deionized_water["Distilled deionized water"]
    CultureMech_008815 --> ing0_Distilled_deionized_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_deionized_water -.-> CHEBI_15377
    ing1_Heart_Infusion_Broth__BD_238400_["Heart Infusion Broth (BD 238400)"]
    CultureMech_008815 --> ing1_Heart_Infusion_Broth__BD_238400_
    ing2_Agar__Noble__BD_214230_["Agar, Noble (BD 214230)"]
    CultureMech_008815 --> ing2_Agar__Noble__BD_214230_
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar__Noble__BD_214230_ -.-> CHEBI_2509
    ing3_Horse_serum["Horse serum"]
    CultureMech_008815 --> ing3_Horse_serum

Ingredients (4)

Preparation

1. Combine Heart Infusion Broth, Agar Noble, and 450.0 ml/L distilled deionized water.
2. Boil to dissolve the Agar Noble.
3. Autoclave the basal agar at 121 C for 15 minutes.
4. Filter-sterilize Sucrose dissolved in 200.0 ml/L water.
5. Heat-inactivate Horse serum at 56 C for 30 minutes.
6. Cool the basal agar to 50-55 C, warm the serum, yeast extract, and sucrose solutions to 50-55 C, and aseptically combine.

Solutions

Target organisms

No target organisms are recorded.

Provenance