Overview
- External ontology
- TOGO:M2228 — Friis medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2228 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008816["friis_medium"]
ing0_Horse_serum["Horse serum"]
CultureMech_008816 --> ing0_Horse_serum
ing1_Swine_serum["Swine serum"]
CultureMech_008816 --> ing1_Swine_serum
ing2_Basal_broth["Basal broth"]
CultureMech_008816 --> ing2_Basal_broth
ing3_Yeast_extract["Yeast extract"]
CultureMech_008816 --> ing3_Yeast_extract
ing4_Bacitracin["Bacitracin"]
CultureMech_008816 --> ing4_Bacitracin
CHEBI_28669["CHEBI:28669"]
ing4_Bacitracin -.-> CHEBI_28669
ing5_Meticillin["Meticillin"]
CultureMech_008816 --> ing5_Meticillin
ing6_Water["Water"]
CultureMech_008816 --> ing6_Water
CHEBI_15377["CHEBI:15377"]
ing6_Water -.-> CHEBI_15377
ing7_Calf_brains["Calf brains"]
CultureMech_008816 --> ing7_Calf_brains
ing8_Beef_heart["Beef heart"]
CultureMech_008816 --> ing8_Beef_heart
ing9_Proteose_peptone["Proteose peptone"]
CultureMech_008816 --> ing9_Proteose_peptone
ing10_Dextrose["Dextrose"]
CultureMech_008816 --> ing10_Dextrose
CHEBI_17634["CHEBI:17634"]
ing10_Dextrose -.-> CHEBI_17634
ing11_Sodium_chloride["Sodium chloride"]
CultureMech_008816 --> ing11_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing11_Sodium_chloride -.-> CHEBI_26710
ing12_Disodium_phosphate["Disodium phosphate"]
CultureMech_008816 --> ing12_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing12_Disodium_phosphate -.-> CHEBI_34683
ing13_Bacto_pleuropneumonia_like_organisms__PPLO__broth_without_crystal_violet["Bacto pleuropneumonia-like organisms (PPLO) broth without crystal v..."]
CultureMech_008816 --> ing13_Bacto_pleuropneumonia_like_organisms__PPLO__broth_without_crystal_violet
ing14_Stock_A["Stock A"]
CultureMech_008816 --> ing14_Stock_A
ing15_Stock_B["Stock B"]
CultureMech_008816 --> ing15_Stock_B
ing16_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_008816 --> ing16_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing16_MgSO4___7H2O -.-> CHEBI_31795
ing17_NaCl["NaCl"]
CultureMech_008816 --> ing17_NaCl
ing17_NaCl -.-> CHEBI_26710
ing18_MgCl2___6H2O["MgCl2 . 6H2O"]
CultureMech_008816 --> ing18_MgCl2___6H2O
CHEBI_86345["CHEBI:86345"]
ing18_MgCl2___6H2O -.-> CHEBI_86345
ing19_KCl["KCl"]
CultureMech_008816 --> ing19_KCl
CHEBI_32588["CHEBI:32588"]
ing19_KCl -.-> CHEBI_32588
ing20_CaCl2__anhydrous_["CaCl2 (anhydrous)"]
CultureMech_008816 --> ing20_CaCl2__anhydrous_
ing21_KH2PO4["KH2PO4"]
CultureMech_008816 --> ing21_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing21_KH2PO4 -.-> CHEBI_63036
ing22_Na2HPO4___12H2O["Na2HPO4 . 12H2O"]
CultureMech_008816 --> ing22_Na2HPO4___12H2O
ing22_Na2HPO4___12H2O -.-> CHEBI_34683
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Horse serum | 40 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 | Swine serum | 40 G_PER_L | Role: Nutrient source | |
| 3 | Basal broth | 320 G_PER_L | ||
| 4 | Yeast extract | 180 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
Bacitracin
C63H98N14O14S
|
800 G_PER_L |
CHEBI:28669
bacitracin
|
Role: Antimicrobial agent; Properties: Defined component, Simple component |
| 6 | Meticillin | 800 G_PER_L | Role: Antimicrobial agent | |
| 7 |
Water
H2O 18.015 g/mol
|
2900.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media [Merged 4 duplicates: 1400.0, 500.0, 500.0, 500.0] |
| 8 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 9 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 10 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 11 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 12 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 13 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 14 | Bacto pleuropneumonia-like organisms (PPLO) broth without crystal violet | 16 G_PER_L | Properties: Undefined component, Complex component | |
| 15 | Stock A | 25 G_PER_L | ||
| 16 | Stock B | 25 G_PER_L | ||
| 17 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 18 |
NaCl
Cl.Na 58.443 g/mol
|
80 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 19 |
MgCl2 . 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 20 |
KCl
Cl.K 74.551 g/mol
|
4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 21 | CaCl2 (anhydrous) | 1.4 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component | |
| 22 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 23 |
Na2HPO4 . 12H2O
HO4P.2Na 141.958 g/mol
|
1.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
Solutions
Phenol red solution
Dose: 10 G_PER_L
Role: pH indicator; Properties: Defined component, Organic compound, Solution
Solution 1
Dose: 557 G_PER_L
See mediadive_6108_Solution_1.yaml for composition
Hanks' balanced salt solution
Dose: 2500 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.180773Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2228 |
| 2026-01-28T19:48:28.725827Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:47.617472+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:18.350459Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:00.459705Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008816 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.133337+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Friis medium" to "friis_medium" for consistent matching |
| 2026-05-16T09:03:12.604394+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.771590+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.726609+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.686353+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:21.891268+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |