m17_medium_supplemented_with_20_g_l_of_lactose

CultureMech:008819 bacterial COMPLEX LIQUID
Original name: M17 medium supplemented with 20 g/L of lactose

Overview

External ontology
TOGO:M2230 — M17 medium supplemented with 20 g/L of lactose
Applications
  • Microbial cultivation
Notes
TOGO M2230 lists M17 medium from Scharlau supplemented with 20 g/L lactose and cultivated anaerobically at 42 C.

Composition

flowchart LR
    CultureMech_008819["m17_medium_supplemented_with_20_g_l_of_lactose"]
    ing0_Lactose["Lactose"]
    CultureMech_008819 --> ing0_Lactose
    CHEBI_36218["CHEBI:36218"]
    ing0_Lactose -.-> CHEBI_36218
    ing1_M17_medium__Scharlau_["M17 medium (Scharlau)"]
    CultureMech_008819 --> ing1_M17_medium__Scharlau_

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Lactose
C12H22O11 342.297 g/mol
20 G_PER_L CHEBI:36218
beta-lactose
TOGO M2230 lists lactose added at 20 g/L.
2 M17 medium (Scharlau) 1000 ML_PER_L TOGO M2230 lists one liter of M17 medium (Scharlau) without disclosing the internal formulation.

Preparation

  1. Supplement one liter of M17 medium (Scharlau) with 20 g/L lactose.
  2. Cultivate anaerobically at 42 C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.184984Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2230
2026-01-28T19:48:35.105756Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.484557Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008819
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.158687+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M17 medium supplemented with 20 g/L of lactose" to "m17_medium_supplemented_with_20_g_l_of_lactose" for consistent matching
2026-06-05T07:41:11.756556+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_togo_m17_bhi_score20.py RESOLVED_TOGO_M17_BHI_SCORE20 TOGO M2230 lists M17 medium from Scharlau supplemented with 20 g/L lactose and cultivated anaerobically at 42 C.