Overview
- External ontology
- TOGO:M2231 — Allen medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2231
Composition
flowchart LR
CultureMech_008820["allen_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_008820 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_Deionized_water["Deionized water"]
CultureMech_008820 --> ing1_Deionized_water
ing2_HEPES_buffer["HEPES buffer"]
CultureMech_008820 --> ing2_HEPES_buffer
CHEBI_46756["CHEBI:46756"]
ing2_HEPES_buffer -.-> CHEBI_46756
ing3_K2HPO4_7H2O__6_g_L_["K2HPO4·7H2O (6 g/L)"]
CultureMech_008820 --> ing3_K2HPO4_7H2O__6_g_L_
ing4_MgSO4_7H2O__6_g_L_["MgSO4·7H2O (6 g/L)"]
CultureMech_008820 --> ing4_MgSO4_7H2O__6_g_L_
ing5_Na2CO3__4_g_L_["Na2CO3 (4 g/L)"]
CultureMech_008820 --> ing5_Na2CO3__4_g_L_
ing6_CaCl2_2H2O__2_5_g_L_["CaCl2·2H2O (2.5 g/L)"]
CultureMech_008820 --> ing6_CaCl2_2H2O__2_5_g_L_
ing7_Na2SiO3_9H2O__4_64_g_L_["Na2SiO3·9H2O (4.64 g/L)"]
CultureMech_008820 --> ing7_Na2SiO3_9H2O__4_64_g_L_
ing8_Citric_acid_H2O__4_8_g_L_["Citric acid·H2O (4.8 g/L)"]
CultureMech_008820 --> ing8_Citric_acid_H2O__4_8_g_L_
ing9_CO2["CO2"]
CultureMech_008820 --> ing9_CO2
CHEBI_16526["CHEBI:16526"]
ing9_CO2 -.-> CHEBI_16526
ing10_Sterile_air["Sterile air"]
CultureMech_008820 --> ing10_Sterile_air
ing11_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008820 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008820 --> ing12_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_4H2O -.-> CHEBI_86368
ing13_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008820 --> ing13_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing13_CoCl2_6H2O -.-> CHEBI_35696
ing14_ZnCl2["ZnCl2"]
CultureMech_008820 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008820 --> ing15_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing15_FeCl3_6H2O -.-> CHEBI_86254
ing16_Na2EDTA_2H2O["Na2EDTA·2H2O"]
CultureMech_008820 --> ing16_Na2EDTA_2H2O
CHEBI_64758["CHEBI:64758"]
ing16_Na2EDTA_2H2O -.-> CHEBI_64758
ing17_K2HPO4_7H2O["K2HPO4·7H2O"]
CultureMech_008820 --> ing17_K2HPO4_7H2O
ing18_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008820 --> ing18_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing18_MgSO4_7H2O -.-> CHEBI_31795
ing19_Na2CO3["Na2CO3"]
CultureMech_008820 --> ing19_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing19_Na2CO3 -.-> CHEBI_29377
ing20_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008820 --> ing20_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing20_CaCl2_2H2O -.-> CHEBI_86158
ing21_Na2SiO3_9H2O["Na2SiO3·9H2O"]
CultureMech_008820 --> ing21_Na2SiO3_9H2O
CHEBI_132108["CHEBI:132108"]
ing21_Na2SiO3_9H2O -.-> CHEBI_132108
ing22_Citric_acid_H2O["Citric acid·H2O"]
CultureMech_008820 --> ing22_Citric_acid_H2O
CHEBI_31404["CHEBI:31404"]
ing22_Citric_acid_H2O -.-> CHEBI_31404
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
1.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Deionized water | 207.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 8 duplicates: 200.0, 1.0, 1.0, 1.0, 1.0, 1.0, 1.0, 1.0] | |
| 3 | HEPES buffer | 2.3 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Defined component, Solution |
| 4 | K2HPO4·7H2O (6 g/L) | 5 G_PER_L | ||
| 5 | MgSO4·7H2O (6 g/L) | 5 G_PER_L | ||
| 6 | Na2CO3 (4 g/L) | 5 G_PER_L | ||
| 7 | CaCl2·2H2O (2.5 g/L) | 10 G_PER_L | ||
| 8 | Na2SiO3·9H2O (4.64 g/L) | 10 G_PER_L | ||
| 9 | Citric acid·H2O (4.8 g/L) | 1 G_PER_L | ||
| 10 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 11 | Sterile air | variable VARIABLE | Properties: Gas | |
| 12 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.004 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.041 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.002 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.005 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.097 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2EDTA·2H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
0.75 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
Properties: Defined component, Organic compound, Simple component |
| 18 | K2HPO4·7H2O | 6 G_PER_L | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component | |
| 19 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2SiO3·9H2O
9H2O.2Na.O3Si 284.198 g/mol
|
4.64 G_PER_L |
CHEBI:132108
sodium silicate nonahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Citric acid·H2O
C6H8O7.H2O 210.138 g/mol
|
4.8 G_PER_L |
CHEBI:31404
Citric acid monohydrate
|
Properties: Defined component, Simple component, Organic compound |
Solutions
P-IV metal solution
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:56.185691Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2231 |
| 2026-01-28T19:48:17.354031Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: Sterile air |
| 2026-03-09T08:04:28.736771+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:57.544695Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:20:00.493090Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008820 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.167346+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Allen medium" to "allen_medium" for consistent matching |
| 2026-05-16T09:03:23.891848+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:48.246910+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:35.805901+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.931635+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |