Overview
- External ontology
- TOGO:M2233 — Frey broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2233
Composition
flowchart LR
CultureMech_008822["frey_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_008822 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008822 --> ing1_Yeast_extract
ing2_Sodium_chloride["Sodium chloride"]
CultureMech_008822 --> ing2_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing2_Sodium_chloride -.-> CHEBI_26710
ing3_Monopotassium_phosphate["Monopotassium phosphate"]
CultureMech_008822 --> ing3_Monopotassium_phosphate
CHEBI_63036["CHEBI:63036"]
ing3_Monopotassium_phosphate -.-> CHEBI_63036
ing4_Potassium_chloride["Potassium chloride"]
CultureMech_008822 --> ing4_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing4_Potassium_chloride -.-> CHEBI_32588
ing5_Disodium_phosphate["Disodium phosphate"]
CultureMech_008822 --> ing5_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Disodium_phosphate -.-> CHEBI_34683
ing6_Magnesium_sulphate["Magnesium sulphate"]
CultureMech_008822 --> ing6_Magnesium_sulphate
ing7_Horse_Serum__RM1239_["Horse Serum (RM1239)"]
CultureMech_008822 --> ing7_Horse_Serum__RM1239_
ing8_Casein_enzymic_hydrolysate["Casein enzymic hydrolysate"]
CultureMech_008822 --> ing8_Casein_enzymic_hydrolysate
ing9_Papaic_digest_of_soyabean_meal["Papaic digest of soyabean meal"]
CultureMech_008822 --> ing9_Papaic_digest_of_soyabean_meal
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Sodium chloride
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Monopotassium phosphate
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
Potassium chloride
Cl.K 74.551 g/mol
|
0.4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
|
1.6 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Magnesium sulphate | 0.2 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 8 | Horse Serum (RM1239) | 100 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 9 | Casein enzymic hydrolysate | 7.5 G_PER_L | Properties: Undefined component | |
| 10 | Papaic digest of soyabean meal | 2.5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.193536Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2233 |
| 2026-01-28T19:48:15.415050Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.522036Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008822 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.197481+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Frey broth" to "frey_broth" for consistent matching |
| 2026-06-05T07:41:05.534382+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |