Overview
- External ontology
- TOGO:M2237 — Basal salts medium (BSM) modified from that of Hareland et al. ( J Bacteriol 121:272– 285(1975)) by removal of the nitriloacetic acid
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2237
Composition
flowchart LR
CultureMech_008825["basal_salts_medium_bsm_modified_from_that_of_hareland_et_al_j_bacte..."]
ing0_Distilled_water["Distilled water"]
CultureMech_008825 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Magnesium_sulfate_heptahydrate["Magnesium sulfate heptahydrate"]
CultureMech_008825 --> ing1_Magnesium_sulfate_heptahydrate
CHEBI_31795["CHEBI:31795"]
ing1_Magnesium_sulfate_heptahydrate -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008825 --> ing2_Yeast_extract
ing3_Ammonium_chloride["Ammonium chloride"]
CultureMech_008825 --> ing3_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing3_Ammonium_chloride -.-> CHEBI_31206
ing4_Ferrous_sulfate_heptahydrate["Ferrous sulfate heptahydrate"]
CultureMech_008825 --> ing4_Ferrous_sulfate_heptahydrate
CHEBI_75836["CHEBI:75836"]
ing4_Ferrous_sulfate_heptahydrate -.-> CHEBI_75836
ing5_Zinc_sulfate_heptahydrate["Zinc sulfate heptahydrate"]
CultureMech_008825 --> ing5_Zinc_sulfate_heptahydrate
CHEBI_32312["CHEBI:32312"]
ing5_Zinc_sulfate_heptahydrate -.-> CHEBI_32312
ing6_Cobalt_sulfate_heptahydrate["Cobalt sulfate heptahydrate"]
CultureMech_008825 --> ing6_Cobalt_sulfate_heptahydrate
ing7_Manganese_sulfate_monohydrate["Manganese sulfate monohydrate"]
CultureMech_008825 --> ing7_Manganese_sulfate_monohydrate
CHEBI_86364["CHEBI:86364"]
ing7_Manganese_sulfate_monohydrate -.-> CHEBI_86364
ing8_di_Potassium_hydrogen_orthophosphate_trihydrate["di‐Potassium hydrogen orthophosphate trihydrate"]
CultureMech_008825 --> ing8_di_Potassium_hydrogen_orthophosphate_trihydrate
ing9_Sodium_dihydrogen_orthophosphate_monohydrate["Sodium dihydrogen orthophosphate monohydrate"]
CultureMech_008825 --> ing9_Sodium_dihydrogen_orthophosphate_monohydrate
ing10_Glucose["Glucose"]
CultureMech_008825 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_CAS_amino_acids["CAS amino acids"]
CultureMech_008825 --> ing11_CAS_amino_acids
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Magnesium sulfate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 | Yeast extract | 50 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
40 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Ferrous sulfate heptahydrate
Fe.7H2O.O4S 278.013 g/mol
|
1.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Zinc sulfate heptahydrate
7H2O.O4S.Zn 287.558 g/mol
|
0.3 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Cobalt sulfate heptahydrate | 0.1 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound | |
| 8 |
Manganese sulfate monohydrate
H2O.Mn.O4S 169.016 g/mol
|
0.3 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 | di‐Potassium hydrogen orthophosphate trihydrate | 85 G_PER_L | Role: Buffer; Properties: Defined component, Simple component, Inorganic compound | |
| 10 | Sodium dihydrogen orthophosphate monohydrate | 20 G_PER_L | Role: Buffer; Properties: Simple component, Inorganic compound, Defined component | |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 12 | CAS amino acids | 50 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.200603Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2237 |
| 2026-01-28T19:48:24.665661Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.552426Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008825 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.228783+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Basal salts medium (BSM) modified from that of Hareland et al. ( J Bacteriol 121:272– 285(1975)) by removal of the nitriloacetic acid" to "basal_salts_medium_bsm_modified_from_that_of_hareland_et_al_j_bacteriol_121_272_285_1975_by_removal_of_the_nitriloacetic_acid" for consistent matching |
| 2026-06-05T07:40:50.562491+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.014302+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |