Overview
- External ontology
- TOGO:M223 — Methanosarcina Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M223 Original source: JCM - JCM_M230 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=230
Composition
flowchart LR
CultureMech_008828["methanosarcina_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008828 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008828 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008828 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008828 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008828 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_008828 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_K2HPO4["K2HPO4"]
CultureMech_008828 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Resazurin["Resazurin"]
CultureMech_008828 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Na2S_9H2O["Na2S・9H2O"]
CultureMech_008828 --> ing8_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_9H2O -.-> CHEBI_76209
ing9_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_008828 --> ing9_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_7H2O -.-> CHEBI_75836
ing10_NaHCO3["NaHCO3"]
CultureMech_008828 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Methanol["Methanol"]
CultureMech_008828 --> ing11_Methanol
CHEBI_17790["CHEBI:17790"]
ing11_Methanol -.-> CHEBI_17790
ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008828 --> ing12_Yeast_extract__BD_Difco_
ing13_Casitone__BD_Difco_["Casitone (BD-Difco)"]
CultureMech_008828 --> ing13_Casitone__BD_Difco_
ing14_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_008828 --> ing14_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing15_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008828 --> ing15_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing15_Carbon_dioxide_gas -.-> CHEBI_16526
ing16_Nitrogen_gas["Nitrogen gas"]
CultureMech_008828 --> ing16_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing16_Nitrogen_gas -.-> CHEBI_17997
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
980 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
2.25 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.227 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.348 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 9 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.85 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 12 |
Methanol
CH4O 32.042 g/mol
|
10 G_PER_L |
CHEBI:17790
methanol
|
Role: Solvating media; Properties: Organic compound, Simple component, Defined component |
| 13 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 14 | Casitone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 15 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 16 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 17 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.650219Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M223 |
| 2026-01-28T19:48:30.090769Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:49.996873+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:00.593983Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008828 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.270265+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanosarcina Medium" to "methanosarcina_medium" for consistent matching |
| 2026-05-16T09:03:12.641389+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:35.808456+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.754530+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.702117+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |