Overview
- External ontology
- TOGO:M2245 — Modified LB medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2245 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008833["modified_lb_medium"]
ing0_NaCl["NaCl"]
CultureMech_008833 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Chloramphenicol["Chloramphenicol"]
CultureMech_008833 --> ing1_Chloramphenicol
CHEBI_17698["CHEBI:17698"]
ing1_Chloramphenicol -.-> CHEBI_17698
ing2_Gentamicin["Gentamicin"]
CultureMech_008833 --> ing2_Gentamicin
CHEBI_17833["CHEBI:17833"]
ing2_Gentamicin -.-> CHEBI_17833
ing3_Agar__BD_["Agar (BD)"]
CultureMech_008833 --> ing3_Agar__BD_
ing4_Tryptone["Tryptone"]
CultureMech_008833 --> ing4_Tryptone
ing5_Yeast_extract["Yeast extract"]
CultureMech_008833 --> ing5_Yeast_extract
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_008833 --> ing6_Sodium_chloride
ing6_Sodium_chloride -.-> CHEBI_26710
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Chloramphenicol
C11H12Cl2N2O5 323.132 g/mol
|
150 G_PER_L |
CHEBI:17698
chloramphenicol
|
Role: Antimicrobial agent; Properties: Defined component, Organic compound, Simple component |
| 3 | Gentamicin | 300 G_PER_L |
CHEBI:17833
gentamycin
|
Role: Antimicrobial agent; Properties: Simple component, Organic compound, Defined component |
| 4 | Agar (BD) | 1.5 PERCENT_W_V | Role: Solidifying component | |
| 5 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 6 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.217088Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2245 |
| 2026-01-28T19:48:14.891295Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.631761Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008833 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.309902+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified LB medium" to "modified_lb_medium" for consistent matching |
| 2026-06-05T07:41:36.787656+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-20T19:02:56.049123+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 2 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |