Overview
- External ontology
- TOGO:M2248 — Luria-Bertani medium supplemented with 50 mM DL-lactate
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2248 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008836["luria_bertani_medium_supplemented_with_50_mm_dl_lactate"]
ing0_DL_lactate["DL-lactate"]
CultureMech_008836 --> ing0_DL_lactate
ing1_Tryptone["Tryptone"]
CultureMech_008836 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_008836 --> ing2_Yeast_extract
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_008836 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Distilled_water["Distilled water"]
CultureMech_008836 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_Yeast_extract["Yeast extract"]
CultureMech_008836 --> ing5_Yeast_extract
ing6_NaCl["NaCl"]
CultureMech_008836 --> ing6_NaCl
ing6_NaCl -.-> CHEBI_26710
ing7_Tryptone["Tryptone"]
CultureMech_008836 --> ing7_Tryptone
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DL-lactate | 50 MILLIMOLAR | Role: Carbon source; Properties: Simple component, Organic compound, Defined component | |
| 2 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 3 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 6 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 | Tryptone | 10 G_PER_L | Properties: Undefined component |
Solutions
NaOH solution
Dose: variable VARIABLE
Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.219521Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2248 |
| 2026-01-28T19:48:09.178869Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH solution |
| 2026-03-09T08:04:14.787299+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:00.643015Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008836 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.320730+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Luria-Bertani medium supplemented with 50 mM DL-lactate" to "luria_bertani_medium_supplemented_with_50_mm_dl_lactate" for consistent matching |
| 2026-05-16T09:03:39.282467+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:11.502548+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |