Overview
- External ontology
- TOGO:M2252 — Artificial seawater base with thiosulfate as the electron donor
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2252 reports a liquid artificial seawater base with 10 mM thiosulfate as the electron donor for microaerobic strain MC-1 growth. Its comments cite PMID:16461683 for the cultivation context and state that cultures were incubated statically under an O2 gradient at 25 C in the dark.
Composition
flowchart LR
CultureMech_008841["artificial_seawater_base_with_thiosulfate_as_the_electron_donor"]
ing0_Artificial_seawater["Artificial seawater"]
CultureMech_008841 --> ing0_Artificial_seawater
ing1_thiosulfate["thiosulfate"]
CultureMech_008841 --> ing1_thiosulfate
CHEBI_26977["CHEBI:26977"]
ing1_thiosulfate -.-> CHEBI_26977
ing2_Oxygen_gas["Oxygen gas"]
CultureMech_008841 --> ing2_Oxygen_gas
CHEBI_15379["CHEBI:15379"]
ing2_Oxygen_gas -.-> CHEBI_15379
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Artificial seawater | 1000.0 ML_PER_L | TOGO M2252 lists 1 L Artificial seawater as the base; this artificial seawater mixture is retained without a single-compound ontology grounding. | |
| 2 | thiosulfate | 10.0 MILLIMOLAR |
CHEBI:26977
thiosulfate
|
TOGO M2252 lists 10 mM thiosulfate as the electron donor. |
| 3 |
Oxygen gas
O2 31.998 g/mol
|
variable VARIABLE |
CHEBI:15379
dioxygen
|
TOGO M2252 lists O2 and states that cultures were incubated statically under an O2 gradient. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.225535Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2252 |
| 2026-01-28T19:48:40.656861Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: O2 |
| 2026-03-13T06:20:00.679832Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008841 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.357685+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Artificial seawater base with thiosulfate as the electron donor" to "artificial_seawater_base_with_thiosulfate_as_the_electron_donor" for consistent matching |
| 2026-05-16T09:03:24.914736+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:49.748748+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m2252_artificial_seawater_thiosulfate_score15.py | RESOLVED_TOGO_M2252_ARTIFICIAL_SEAWATER_THIOSULFATE_SCORE15 | Corrected Artificial seawater from TOGO's imported 1 g/L row to a 1000.0 ml/L base, grounded thiosulfate and O2, added the 25 C static O2-gradient incubation condition, and retained Artificial seawater as intentionally unmapped. |