strength_marine_medium

CultureMech:008842 bacterial COMPLEX SOLID_AGAR
Original name: ½ Strength Marine Medium

Overview

External ontology
TOGO:M2255 — ½ Strength Marine Medium
Applications
  • Microbial cultivation
Notes
TOGO M2255 cites ATCC Medium 2515 1/2 Strength Marine Medium, which lists 18.7 g Marine Broth 2216 from BD 279110, optional 15.0 g Agar, and 1000.0 ml DI Water. The source autoclaves the medium at 121 C and keeps it spinning while dispensing so that precipitate is evenly distributed.

Composition

flowchart LR
    CultureMech_008842["strength_marine_medium"]
    ing0_Marine_Broth_2216__BD_279110_["Marine Broth 2216 (BD 279110)"]
    CultureMech_008842 --> ing0_Marine_Broth_2216__BD_279110_
    ing1_Agar["Agar"]
    CultureMech_008842 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509
    ing2_DI_Water["DI Water"]
    CultureMech_008842 --> ing2_DI_Water
    CHEBI_15377["CHEBI:15377"]
    ing2_DI_Water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Marine Broth 2216 (BD 279110) 18.7 G_PER_L TOGO M2255 / ATCC Medium 2515 lists 18.7 g/L Marine Broth 2216 from BD 279110; this commercial marine broth is retained as an opaque complex component.
2 Agar 15.0 G_PER_L CHEBI:2509
agar
 TOGO M2255 / ATCC Medium 2515 lists 15.0 g/L Agar if solid medium is needed.
3 DI Water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 TOGO M2255 / ATCC Medium 2515 lists 1000.0 ml/L DI Water.

Preparation

  1. Suspend Marine Broth 2216 and Agar, if needed, in 1000 ml DI Water.
  2. Autoclave at 121 C and keep the medium spinning while dispensing so precipitate is evenly distributed.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.226290Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2255
2026-01-28T19:48:37.291817Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.682752Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008842
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.360942+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "½ Strength Marine Medium" to "strength_marine_medium" for consistent matching
2026-06-05T07:41:47.054921+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2255_half_marine_score15.py RESOLVED_TOGO_M2255_HALF_MARINE_SCORE15 TOGO M2255 cites ATCC Medium 2515 1/2 Strength Marine Medium, which lists 18.7 g Marine Broth 2216 from BD 279110, optional 15.0 g Agar, and 1000.0 ml DI Water. The source autoclaves the medium at 121 C and keeps it spinning while dispensing so that precipitate is evenly distributed. Corrected the DI Water unit to ml/L, removed the false kg_microbe_match to DSMZ Soil Extract Medium, and linked this recipe to Marine Broth 2216 as a half-strength concentration variant.