Overview
- External ontology
- TOGO:M2256 — Columbia broth supplemented with 0.01% 3-NAD and 25 mM CaCl2
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2256
Composition
flowchart LR
CultureMech_008843["columbia_broth_supplemented_with_0_01_3_nad_and_25_mm_cacl2"]
ing0_Distilled_water["Distilled water"]
CultureMech_008843 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2["CaCl2"]
CultureMech_008843 --> ing1_CaCl2
CHEBI_3312["CHEBI:3312"]
ing1_CaCl2 -.-> CHEBI_3312
ing2___NAD["β-NAD"]
CultureMech_008843 --> ing2___NAD
ing3_Columbia_broth["Columbia broth"]
CultureMech_008843 --> ing3_Columbia_broth
ing4_Yeast_Extract["Yeast Extract"]
CultureMech_008843 --> ing4_Yeast_Extract
ing5_Sodium_Chloride["Sodium Chloride"]
CultureMech_008843 --> ing5_Sodium_Chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_Chloride -.-> CHEBI_26710
ing6_Dextrose["Dextrose"]
CultureMech_008843 --> ing6_Dextrose
CHEBI_17634["CHEBI:17634"]
ing6_Dextrose -.-> CHEBI_17634
ing7_Sodium_Carbonate["Sodium Carbonate"]
CultureMech_008843 --> ing7_Sodium_Carbonate
CHEBI_29377["CHEBI:29377"]
ing7_Sodium_Carbonate -.-> CHEBI_29377
ing8_Tris__Hydroxymethyl__Aminomethane["Tris (Hydroxymethyl) Aminomethane"]
CultureMech_008843 --> ing8_Tris__Hydroxymethyl__Aminomethane
ing9_Ferrous_Sulfate["Ferrous Sulfate"]
CultureMech_008843 --> ing9_Ferrous_Sulfate
CHEBI_75832["CHEBI:75832"]
ing9_Ferrous_Sulfate -.-> CHEBI_75832
ing10_Magnesium_Sulfate__anhydrous_["Magnesium Sulfate (anhydrous)"]
CultureMech_008843 --> ing10_Magnesium_Sulfate__anhydrous_
ing11_Tris__Hydroxymethyl__Aminomethane_HCl["Tris (Hydroxymethyl) Aminomethane HCl"]
CultureMech_008843 --> ing11_Tris__Hydroxymethyl__Aminomethane_HCl
ing12_Proteose_Peptone_No__3["Proteose Peptone No. 3"]
CultureMech_008843 --> ing12_Proteose_Peptone_No__3
ing13_Pancreatic_Digest_of_Casein["Pancreatic Digest of Casein"]
CultureMech_008843 --> ing13_Pancreatic_Digest_of_Casein
ing14_L_Cysteine_HCl["L-Cysteine HCl"]
CultureMech_008843 --> ing14_L_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing14_L_Cysteine_HCl -.-> CHEBI_91247
ing15_Tryptic_Digest_of_Beef_Heart["Tryptic Digest of Beef Heart"]
CultureMech_008843 --> ing15_Tryptic_Digest_of_Beef_Heart
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
CaCl2
Ca.2Cl 110.984 g/mol
|
25 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | β-NAD | 0.01 PERCENT_W_V | Role: Growth factor; Properties: Defined component, Organic compound, Simple component | |
| 4 | Columbia broth | 35 G_PER_L | ||
| 5 | Yeast Extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
Sodium Chloride
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Dextrose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 8 |
Sodium Carbonate
CO3.2Na 105.988 g/mol
|
0.6 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 9 | Tris (Hydroxymethyl) Aminomethane | 0.83 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 10 |
Ferrous Sulfate
Fe.O4S 151.908 g/mol
|
0.02 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Magnesium Sulfate (anhydrous) | 0.1 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 12 | Tris (Hydroxymethyl) Aminomethane HCl | 2.86 G_PER_L | Role: Buffer | |
| 13 | Proteose Peptone No. 3 | 5 G_PER_L | Properties: Undefined component | |
| 14 | Pancreatic Digest of Casein | 10 G_PER_L | Properties: Undefined component | |
| 15 |
L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.1 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
Properties: Organic compound, Simple component, Defined component |
| 16 | Tryptic Digest of Beef Heart | 3 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.227069Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2256 |
| 2026-01-28T19:48:21.107909Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.688617Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008843 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.367244+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Columbia broth supplemented with 0.01% 3-NAD and 25 mM CaCl2" to "columbia_broth_supplemented_with_0_01_3_nad_and_25_mm_cacl2" for consistent matching |
| 2026-06-05T07:40:54.701832+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |