lb_medium_difco

CultureMech:008857 bacterial COMPLEX LIQUID
Original name: LB medium (Difco)

Overview

External ontology
TOGO:M2270 — LB medium (Difco)
Applications
  • Microbial cultivation
Notes
TOGO M2270 reports LB medium (Difco) with 5 g/L Yeast Extract, 10 g/L Sodium Chloride, and 10 g/L Tryptone.

Composition

flowchart LR
    CultureMech_008857["lb_medium_difco"]
    ing0_Yeast_Extract["Yeast Extract"]
    CultureMech_008857 --> ing0_Yeast_Extract
    ing1_Sodium_Chloride["Sodium Chloride"]
    CultureMech_008857 --> ing1_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing1_Sodium_Chloride -.-> CHEBI_26710
    ing2_Tryptone["Tryptone"]
    CultureMech_008857 --> ing2_Tryptone

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Yeast Extract 5 G_PER_L FOODON:03315426
yeast extract
TOGO M2270 lists 5 g/L Yeast Extract.
2 Sodium Chloride
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
TOGO M2270 lists 10 g/L Sodium Chloride.
3 Tryptone 10 G_PER_L TOGO M2270 lists Tryptone as a digest product not reducible to one ChEBI molecule.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.250050Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2270
2026-01-28T19:48:22.860795Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.793637Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008857
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.479434+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB medium (Difco)" to "lb_medium_difco" for consistent matching
2026-06-05T07:41:10.913319+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2270_m2910_lb_difco_lennox_score15.py RESOLVED_TOGO_M2270_M2910_LB_DIFCO_LENNOX_SCORE15 TOGO M2270 reports LB medium (Difco) with 5 g/L Yeast Extract, 10 g/L Sodium Chloride, and 10 g/L Tryptone. Added explicit FoodOn/ChEBI grounding for the two mappable components while leaving tryptone ungrounded as an undefined digest product.