Overview
- External ontology
- TOGO:M2273 — Marine-Caulobacter medium SPYEM containing sea salts and NH4Cl
- Applications
-
- Microbial cultivation
- Notes
- TOGO Medium M2273 lists an autoclaved base of 0.5 g NH4Cl, 30 g Sea salts (Sigma), and 1 L deionized water. After autoclaving and cooling, TOGO adds 2 ml sterile 50% glucose, 20 ml 50xPYE, and 5 ml filter-sterilized 0.2 mg/ml riboflavin per liter of medium; 50xPYE contains 100 g peptone and 50 g yeast extract in 1 L deionized water and is autoclaved.
Composition
flowchart LR
CultureMech_008859["marine_caulobacter_medium_spyem_containing_sea_salts_and_nh4cl"]
ing0_NH4Cl["NH4Cl"]
CultureMech_008859 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_Sea_salts__Sigma_["Sea salts (Sigma)"]
CultureMech_008859 --> ing1_Sea_salts__Sigma_
ing2_Deionized_water["Deionized water"]
CultureMech_008859 --> ing2_Deionized_water
CHEBI_15377["CHEBI:15377"]
ing2_Deionized_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
TOGO M2273 lists 0.5 g/L NH4Cl in the autoclaved base. |
| 2 | Sea salts (Sigma) | 30 G_PER_L | TOGO M2273 lists 30 g/L Sea salts from Sigma without disclosing the product composition. | |
| 3 |
Deionized water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
TOGO M2273 lists 1 L deionized water in the autoclaved base. |
Preparation
- Mix 0.5 g NH4Cl and 30 g Sea salts (Sigma) in 1 L deionized water.
- Autoclave the base medium and cool it.
- Autoclave 50xPYE before use.
- Filter-sterilize the 0.2 mg/ml riboflavin solution.
- Aseptically add 20 ml 50xPYE, 2 ml sterile 50% glucose, and 5 ml filter-sterilized 0.2 mg/ml riboflavin per liter.
Solutions
Glucose (50%)
Dose: 2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 50 PERCENT_W_V |
TOGO M2273 adds 2 ml/L sterile 50% glucose after cooling.
50xPYE
Dose: 20 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Peptone | 100 G_PER_L | |
| Yeast extract | 50 G_PER_L | |
| Deionized water | 1000 ML_PER_L |
TOGO M2273 adds 20 ml/L autoclaved 50xPYE containing 100 g/L peptone and 50 g/L yeast extract.
Riboflavin (0.2 mg/ml)
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Riboflavin | 0.2 MG_PER_ML | |
| Deionized water | 10 ML_PER_L |
TOGO M2273 adds 5 ml/L filter-sterilized 0.2 mg/ml riboflavin solution after cooling.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.252249Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2273 |
| 2026-01-28T19:48:11.692466Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:46.981780Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:00.803903Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008859 |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.489863+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Marine-Caulobacter medium SPYEM containing sea salts and NH4Cl" to "marine_caulobacter_medium_spyem_containing_sea_salts_and_nh4cl" for consistent matching |
| 2026-06-05T07:41:12.879339+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.880820+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m2273_marine_caulobacter_score15.py | RESOLVED_TOGO_M2273_MARINE_CAULOBACTER_SCORE15 | TOGO Medium M2273 lists an autoclaved base of 0.5 g NH4Cl, 30 g Sea salts (Sigma), and 1 L deionized water. After autoclaving and cooling, TOGO adds 2 ml sterile 50% glucose, 20 ml 50xPYE, and 5 ml filter-sterilized 0.2 mg/ml riboflavin per liter of medium; 50xPYE contains 100 g peptone and 50 g yeast extract in 1 L deionized water and is autoclaved. Restored top-level deionized water to 1 L, converted glucose, 50xPYE, and riboflavin from g/L ingredient rows to ml/L stock-solution additions, and moved stock-only peptone, yeast extract, riboflavin, and deionized-water rows into nested stock compositions. |