pfennig_s_medium

CultureMech:008863 bacterial COMPLEX LIQUID
Original name: Pfennig’s medium

Overview

External ontology
TOGO:M2277 — Pfennig’s medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2277

Composition

flowchart LR
    CultureMech_008863["pfennig_s_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008863 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4.7H2O"]
    CultureMech_008863 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2.2H2O"]
    CultureMech_008863 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008863 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_008863 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Na2MoO4_2H2O["Na2MoO4.2H2O"]
    CultureMech_008863 --> ing5_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing5_Na2MoO4_2H2O -.-> CHEBI_75213
    ing6_H3BO3["H3BO3"]
    CultureMech_008863 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_FeSO4_7H2O["FeSO4.7H2O"]
    CultureMech_008863 --> ing7_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_7H2O -.-> CHEBI_75836
    ing8_MnCI2_4H2O["MnCI2.4H2O"]
    CultureMech_008863 --> ing8_MnCI2_4H2O
    ing9_CoCI2_6H2O["CoCI2.6H2O"]
    CultureMech_008863 --> ing9_CoCI2_6H2O
    ing10_NiCI2_6H2O["NiCI2.6H2O"]
    CultureMech_008863 --> ing10_NiCI2_6H2O
    ing11_CuCl2_2H2O["CuCl2.2H2O"]
    CultureMech_008863 --> ing11_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_2H2O -.-> CHEBI_86318
    ing12_ZnCI2["ZnCI2"]
    CultureMech_008863 --> ing12_ZnCI2
    ing13_Ethylenediamine_tetraacetate_di_Na_salt["Ethylenediamine-tetraacetate-di-Na-salt"]
    CultureMech_008863 --> ing13_Ethylenediamine_tetraacetate_di_Na_salt
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_008863 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1051.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 950.0, 1.0, 100.0]
2 MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2.2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6 Na2MoO4.2H2O
2H2O.MoO4.2Na 241.946 g/mol
18 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 H3BO3
H3BO3 61.833 g/mol
300 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 MnCI2.4H2O 50 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 CoCI2.6H2O 190 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 NiCI2.6H2O 24 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 CuCl2.2H2O
Cl2Cu.2H2O 170.482 g/mol
2 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 ZnCI2 42 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 Ethylenediamine-tetraacetate-di-Na-salt 3 G_PER_L Properties: Defined component, Organic compound, Simple component
15 Vitamin B12 2 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

Solution 1

Dose: 951 G_PER_L

See mediadive_6108_Solution_1.yaml for composition

Solution 3: Vitamin B12 solution

Dose: 1 G_PER_L

Solution 4: Na bicarbonate solution

Dose: 30 G_PER_L

Solution 5: Sodium sulfide solution

Dose: 10 G_PER_L

Solution 2 (SL 12)

Dose: 1 G_PER_L

5% Na bicarbonate solution

Dose: 30 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

6% Sodium sulfide solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.255854Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2277
2026-01-28T19:48:12.656879Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:20.918661+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 7 solution(s) from ingredients
2026-03-13T03:08:48.953252Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:00.821142Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008863
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.507602+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Pfennig’s medium" to "pfennig_s_medium" for consistent matching
2026-05-16T09:04:28.867288+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:41.528702+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.103536+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.