Overview
- External ontology
- TOGO:M228 — Thermocladium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M228 Original source: JCM - JCM_M236 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=236
Composition
flowchart LR
CultureMech_008876["thermocladium_medium"]
ing0_Resazurin["Resazurin"]
CultureMech_008876 --> ing0_Resazurin
CHEBI_8806["CHEBI:8806"]
ing0_Resazurin -.-> CHEBI_8806
ing1_Na2S_9H2O["Na2S・9H2O"]
CultureMech_008876 --> ing1_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing1_Na2S_9H2O -.-> CHEBI_76209
ing2_Sulfur__powder_["Sulfur (powder)"]
CultureMech_008876 --> ing2_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing2_Sulfur__powder_ -.-> CHEBI_33403
ing3_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
CultureMech_008876 --> ing3_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing3_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008876 --> ing4_Yeast_extract__BD_Difco_
ing5_H2SO4["H2SO4"]
CultureMech_008876 --> ing5_H2SO4
CHEBI_26836["CHEBI:26836"]
ing5_H2SO4 -.-> CHEBI_26836
ing6_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008876 --> ing6_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing6_Carbon_dioxide_gas -.-> CHEBI_16526
ing7_N2["N2"]
CultureMech_008876 --> ing7_N2
CHEBI_17997["CHEBI:17997"]
ing7_N2 -.-> CHEBI_17997
ing8_Hydrogen_gas["Hydrogen gas"]
CultureMech_008876 --> ing8_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing8_Hydrogen_gas -.-> CHEBI_18276
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 2 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
|
2.94 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 5 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 6 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 7 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 8 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 9 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
Modified Brock's salt base solution (see Medium [M156])
Dose: 1 G_PER_L
Cross-reference to Medium M156
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:54.660823Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M228 |
| 2026-01-28T19:48:13.799174Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:22.869791+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:00.952948Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008876 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.647214+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Thermocladium Medium" to "thermocladium_medium" for consistent matching |
| 2026-05-16T09:03:12.857625+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.039324+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |