heart_infusion_agar_supplemented_with_5_sheep_blood

CultureMech:008877 bacterial COMPLEX SOLID_AGAR
Original name: Heart Infusion Agar (supplemented with 5% sheep blood)

Overview

External ontology
TOGO:M2291 — Heart Infusion Agar (supplemented with 5% sheep blood)
Applications
  • Microbial cultivation
Notes
TOGO M2291 reports routine growth on Heart Infusion Agar (Difco) supplemented with 5% sheep blood (Oxoid) for 2 days at 37 C in an incubator containing 5% CO2. CO2 is retained as the incubation aeration condition instead of as a medium ingredient.

Composition

flowchart LR
    CultureMech_008877["heart_infusion_agar_supplemented_with_5_sheep_blood"]
    ing0_Heart_Infusion_Agar__Difco_["Heart Infusion Agar (Difco)"]
    CultureMech_008877 --> ing0_Heart_Infusion_Agar__Difco_
    ing1_Sheep_blood["Sheep blood"]
    CultureMech_008877 --> ing1_Sheep_blood

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Heart Infusion Agar (Difco) 95 PERCENT_V_V TOGO M2291 lists the commercial Difco Heart Infusion Agar base with 5% sheep blood as the final supplement; the base is the remaining 95% v/v and is retained as an opaque component.
2 Sheep blood 5 PERCENT_V_V UBERON:0000178
blood
 TOGO M2291 lists 5% sheep blood from Oxoid as the supplement.

Preparation

  1. Supplement Heart Infusion Agar (Difco) with 5% sheep blood (Oxoid).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.284965Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2291
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2; Added default concentration for ingredient: Heart Infusion Agar (Difco)
2026-03-13T06:20:00.957092Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008877
2026-03-17T19:46:19.651612+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Heart Infusion Agar (supplemented with 5% sheep blood)" to "heart_infusion_agar_supplemented_with_5_sheep_blood" for consistent matching
2026-05-16T09:03:37.045379+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-09-12T00:00:00-07:00 repair_togo_m2291_heart_infusion_sheep_score15.py RESOLVED_TOGO_M2291_HEART_INFUSION_SHEEP_SCORE15 TOGO M2291 reports routine growth on Heart Infusion Agar (Difco) supplemented with 5% sheep blood (Oxoid) for 2 days at 37 C in an incubator containing 5% CO2. CO2 is retained as the incubation aeration condition instead of as a medium ingredient. Corrected sheep blood from %w/v to %v/v, grounded it to UBERON blood, represented the prepared Difco agar base as the remaining 95% v/v, removed CO2 from the ingredients, and kept Heart Infusion Agar (Difco) intentionally unmapped as a source-disclosed commercial base.