Overview
- External ontology
- TOGO:M2304 — M-R2A medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2304 Original URL: https://www.atcc.org/~/media/8DE5B30C824649A0BB417654D8A758BF.ashx
Composition
flowchart LR
CultureMech_008890["m_r2a_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008890 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008890 --> ing1_Yeast_extract
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008890 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008890 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008890 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_008890 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Dextrose["Dextrose"]
CultureMech_008890 --> ing6_Dextrose
CHEBI_17634["CHEBI:17634"]
ing6_Dextrose -.-> CHEBI_17634
ing7_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008890 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008890 --> ing8_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_6H2O -.-> CHEBI_86345
ing9_H3BO3["H3BO3"]
CultureMech_008890 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008890 --> ing10_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_7H2O -.-> CHEBI_75836
ing11_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008890 --> ing11_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_4H2O -.-> CHEBI_86368
ing12_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008890 --> ing12_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing12_CoCl2_6H2O -.-> CHEBI_35696
ing13_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008890 --> ing13_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_2H2O -.-> CHEBI_86318
ing14_ZnCl2["ZnCl2"]
CultureMech_008890 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_Na2SO4["Na2SO4"]
CultureMech_008890 --> ing15_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing15_Na2SO4 -.-> CHEBI_32149
ing16_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008890 --> ing16_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing16_Sodium_pyruvate -.-> CHEBI_50144
ing17_KNO3["KNO3"]
CultureMech_008890 --> ing17_KNO3
CHEBI_63043["CHEBI:63043"]
ing17_KNO3 -.-> CHEBI_63043
ing18_NiSO4_6H2O["NiSO4·6H2O"]
CultureMech_008890 --> ing18_NiSO4_6H2O
CHEBI_53001["CHEBI:53001"]
ing18_NiSO4_6H2O -.-> CHEBI_53001
ing19_Soluble_starch["Soluble starch"]
CultureMech_008890 --> ing19_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing19_Soluble_starch -.-> CHEBI_28017
ing20_Agar["Agar"]
CultureMech_008890 --> ing20_Agar
CHEBI_2509["CHEBI:2509"]
ing20_Agar -.-> CHEBI_2509
ing21_Casamino_acids["Casamino acids"]
CultureMech_008890 --> ing21_Casamino_acids
ing22_Peptone["Peptone"]
CultureMech_008890 --> ing22_Peptone
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.25 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.8 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Dextrose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 8 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
10 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
7 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.5 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.3 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.5 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2SO4
2Na.O4S 142.043 g/mol
|
5 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 18 |
KNO3
K.NO3 101.102 g/mol
|
0.505 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiSO4·6H2O
Ni.O4S 154.756 g/mol
|
0.5 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 21 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 22 | Casamino acids | 0.5 G_PER_L | Properties: Undefined component | |
| 23 | Peptone | 0.5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.306913Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2304 |
| 2026-01-28T19:48:16.221836Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:01.071947Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008890 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.773344+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M-R2A medium" to "m_r2a_medium" for consistent matching |
| 2026-06-05T07:41:12.415438+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.595828+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:00.756354+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |