Overview
- External ontology
- TOGO:M2307 — Bordet Gengou Agar Medium
- Applications
-
- Microbial cultivation
- pH
- 6.7
- Notes
- TOGO M2307 imports ATCC Medium 35 Bordet Gengou Agar Medium. ATCC Medium 35 prepares the agar from 30.0 g Bordet-Gengou Agar Base (BD 248200), 10.0 ml glycerol, 10.0 g Proteose Peptone, and 840 ml DI Water, adjusts to pH 6.7 +/- 0.2, autoclaves at 121 C, cools the base to 45-50 C, and adds 150 ml sterile defibrinated rabbit blood.
Composition
flowchart LR
CultureMech_008893["bordet_gengou_agar_medium"]
ing0_Bordet_Gengou_Agar_Base__BD_248200_["Bordet-Gengou Agar Base (BD 248200)"]
CultureMech_008893 --> ing0_Bordet_Gengou_Agar_Base__BD_248200_
ing1_Glycerol["Glycerol"]
CultureMech_008893 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_Proteose_Peptone["Proteose Peptone"]
CultureMech_008893 --> ing2_Proteose_Peptone
ing3_DI_Water["DI Water"]
CultureMech_008893 --> ing3_DI_Water
CHEBI_15377["CHEBI:15377"]
ing3_DI_Water -.-> CHEBI_15377
ing4_Sterile_Defibrinated_Rabbit_Blood["Sterile Defibrinated Rabbit Blood"]
CultureMech_008893 --> ing4_Sterile_Defibrinated_Rabbit_Blood
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bordet-Gengou Agar Base (BD 248200) | 30.0 G_PER_L | ATCC Medium 35 lists 30.0 g/L commercial Bordet-Gengou Agar Base (BD 248200). | |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
10.0 ML_PER_L |
CHEBI:17754
glycerol
|
ATCC Medium 35 lists 10.0 ml/L glycerol. |
| 3 | Proteose Peptone | 10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
Proteose Peptone
|
ATCC Medium 35 lists 10.0 g/L Proteose Peptone. |
| 4 |
DI Water
H2O 18.015 g/mol
|
840.0 ML_PER_L |
CHEBI:15377
water
|
ATCC Medium 35 lists 840 ml/L DI Water in the agar base. |
| 5 | Sterile Defibrinated Rabbit Blood | 15 PERCENT_V_V |
UBERON:0000178
blood
|
ATCC Medium 35 lists 150 ml sterile defibrinated rabbit blood per liter. |
Preparation
- Suspend Bordet-Gengou Agar Base, glycerol, and Proteose Peptone in 840 ml DI Water.
- Adjust to pH 6.7 +/- 0.2.
- Autoclave the base at 121 C.
- Cool the base to 45-50 C and add 150 ml sterile defibrinated rabbit blood.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.315144Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2307 |
| 2026-01-28T19:48:33.058959Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:01.102207Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008893 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.805022+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Bordet Gengou Agar Medium" to "bordet_gengou_agar_medium" for consistent matching |
| 2026-06-05T07:40:51.294623+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.034502+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-11T00:00:00-07:00 | repair_togo_m2307_m2308_bordet_atcc_score15.py | RESOLVED_TOGO_M2307_M2308_BORDET_ATCC_SCORE15 | TOGO M2307 imports ATCC Medium 35 Bordet Gengou Agar Medium. ATCC Medium 35 prepares the agar from 30.0 g Bordet-Gengou Agar Base (BD 248200), 10.0 ml glycerol, 10.0 g Proteose Peptone, and 840 ml DI Water, adjusts to pH 6.7 +/- 0.2, autoclaves at 121 C, cools the base to 45-50 C, and adds 150 ml sterile defibrinated rabbit blood. |