bordet_gengou_broth_medium

CultureMech:008894 bacterial COMPLEX LIQUID
Original name: Bordet Gengou Broth Medium

Overview

External ontology
TOGO:M2308 — Bordet Gengou Broth Medium
Applications
  • Microbial cultivation
pH
6.7
Notes
TOGO M2308 imports ATCC Medium 35 Bordet Gengou Broth Medium. ATCC Medium 35 prepares the broth from 10.0 ml glycerol, 10.0 g Proteose Peptone, 840 ml Bordet-Gengou Broth Base, and 150 ml sterile defibrinated rabbit blood. The broth base is prepared from 125 g potato, 5.5 g NaCl, and DI Water brought back to 1000 ml.

Composition

flowchart LR
    CultureMech_008894["bordet_gengou_broth_medium"]
    ing0_Glycerol["Glycerol"]
    CultureMech_008894 --> ing0_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing0_Glycerol -.-> CHEBI_17754
    ing1_Proteose_Peptone["Proteose Peptone"]
    CultureMech_008894 --> ing1_Proteose_Peptone
    ing2_Sterile_Defibrinated_Rabbit_Blood["Sterile Defibrinated Rabbit Blood"]
    CultureMech_008894 --> ing2_Sterile_Defibrinated_Rabbit_Blood

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Glycerol
C3H8O3 92.094 g/mol
10.0 ML_PER_L CHEBI:17754
glycerol
 ATCC Medium 35 lists 10.0 ml/L glycerol.
2 Proteose Peptone 10.0 G_PER_L MICRO:0000180 (source link unavailable)
Proteose Peptone
 ATCC Medium 35 lists 10.0 g/L Proteose Peptone.
3 Sterile Defibrinated Rabbit Blood 15 PERCENT_V_V UBERON:0000178
blood
 ATCC Medium 35 lists 150 ml sterile defibrinated rabbit blood per liter.

Preparation

  1. Mix glycerol and Proteose Peptone into 840 ml prepared Bordet-Gengou Broth Base.
  2. Adjust to pH 6.7 +/- 0.2.
  3. Autoclave the base at 121 C.
  4. Cool the base to 45-50 C and add 150 ml sterile defibrinated rabbit blood.

Solutions

Bordet-Gengou Broth Base

Dose: 840.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Potato 125.0 G_PER_L ATCC Medium 35 boils 125 g potato while preparing Bordet-Gengou Broth Base.
NaCl 5.5 G_PER_L ATCC Medium 35 lists 5.5 g/L NaCl in Bordet-Gengou Broth Base.
DI Water 1000.0 ML_PER_L ATCC Medium 35 brings Bordet-Gengou Broth Base back to 1000 ml.
ATCC Medium 35 adds 840 ml/L prepared Bordet-Gengou Broth Base.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.316088Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2308
2026-01-28T19:48:30.417910Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.106160Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008894
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.809381+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Bordet Gengou Broth Medium" to "bordet_gengou_broth_medium" for consistent matching
2026-06-05T07:40:51.299044+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:36.038992+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-09-11T00:00:00-07:00 repair_togo_m2307_m2308_bordet_atcc_score15.py RESOLVED_TOGO_M2307_M2308_BORDET_ATCC_SCORE15 TOGO M2308 imports ATCC Medium 35 Bordet Gengou Broth Medium. ATCC Medium 35 prepares the broth from 10.0 ml glycerol, 10.0 g Proteose Peptone, 840 ml Bordet-Gengou Broth Base, and 150 ml sterile defibrinated rabbit blood. The broth base is prepared from 125 g potato, 5.5 g NaCl, and DI Water brought back to 1000 ml.