Overview
- External ontology
- TOGO:M230 — Artificial Soil Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M230 Original source: JCM - JCM_M238 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=238
Composition
flowchart LR
CultureMech_008896["artificial_soil_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_008896 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008896 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008896 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_008896 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_NaHCO3["NaHCO3"]
CultureMech_008896 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_008896 --> ing5_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_6H2O -.-> CHEBI_86254
ing6_CaSO4_2H2O["CaSO4・2H2O"]
CultureMech_008896 --> ing6_CaSO4_2H2O
CHEBI_32583["CHEBI:32583"]
ing6_CaSO4_2H2O -.-> CHEBI_32583
ing7_Ca_NO3_2_4H2O["Ca(NO3)2・4H2O"]
CultureMech_008896 --> ing7_Ca_NO3_2_4H2O
CHEBI_86159["CHEBI:86159"]
ing7_Ca_NO3_2_4H2O -.-> CHEBI_86159
ing8_K2SO4["K2SO4"]
CultureMech_008896 --> ing8_K2SO4
CHEBI_32036["CHEBI:32036"]
ing8_K2SO4 -.-> CHEBI_32036
ing9_Glucose["Glucose"]
CultureMech_008896 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_Agar["Agar"]
CultureMech_008896 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_Soil_extract__see_below_["Soil extract (see below)"]
CultureMech_008896 --> ing11_Soil_extract__see_below_
ing12_tap_water["tap water"]
CultureMech_008896 --> ing12_tap_water
ing12_tap_water -.-> CHEBI_15377
ing13_leafy_soil["leafy soil"]
CultureMech_008896 --> ing13_leafy_soil
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
950 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
1 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
CaSO4・2H2O
Ca.2H2O.O4S 172.171 g/mol
|
1.01 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
Ca(NO3)2・4H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.49 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 9 |
K2SO4
2K.O4S 174.259 g/mol
|
25 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 11 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 12 | Soil extract (see below) | 50 G_PER_L | ||
| 13 |
tap water
H2O 18.015 g/mol
|
variable VARIABLE |
CHEBI:15377
water
|
Role: Solvating media; Properties: Undefined component |
| 14 | leafy soil | variable VARIABLE | Role: Growth factor; Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.665713Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M230 |
| 2026-01-28T19:48:08.991613Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: tap water; Added default concentration for ingredient: leafy soil |
| 2026-03-13T06:20:01.121752Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008896 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.825438+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Artificial Soil Agar" to "artificial_soil_agar" for consistent matching |
| 2026-05-16T09:03:12.980844+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.175624+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:26.097565+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.832757+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |