reactivation_with_liquid_medium_464_plate_count_agar

CultureMech:008897 bacterial COMPLEX SOLID_AGAR
Original name: Reactivation With Liquid Medium 464 (Plate Count Agar)

Overview

External ontology
TOGO:M2310 — Reactivation With Liquid Medium 464 (Plate Count Agar)
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M2310 imports DSMZ Medium 464a Reactivation With Liquid Medium 464, which uses DSMZ Medium 464 Plate Count Agar with 5 g tryptone, 2.5 g yeast extract, 1 g dextrose, 15 g agar if required, 1000 ml distilled water, pH 7.0, and rehydration of lyophilized cells from the ampoule in liquid medium 464 before subsequent subculture in liquid or agar medium.

Composition

flowchart LR
    CultureMech_008897["reactivation_with_liquid_medium_464_plate_count_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008897 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008897 --> ing1_Yeast_extract
    ing2_Dextrose["Dextrose"]
    CultureMech_008897 --> ing2_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing2_Dextrose -.-> CHEBI_17634
    ing3_Agar__if_required["Agar, if required"]
    CultureMech_008897 --> ing3_Agar__if_required
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_required -.-> CHEBI_2509
    ing4_Tryptone["Tryptone"]
    CultureMech_008897 --> ing4_Tryptone

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M2310 / DSMZ Medium 464a lists 1000 ml distilled water as the Plate Count Agar solvent; the TOGO import unit is corrected to 1 L.
2 Yeast extract 2.5 G_PER_L FOODON:03315426
Yeast extract
 TOGO M2310 / DSMZ Medium 464a lists 2.5 g/L Yeast extract.
3 Dextrose
C6H12O6 180.156 g/mol
1.0 G_PER_L CHEBI:17634
D-glucose
 TOGO M2310 / DSMZ Medium 464a lists 1 g/L Dextrose.
4 Agar, if required 15.0 G_PER_L CHEBI:2509
agar
 TOGO M2310 / DSMZ Medium 464a lists 15 g/L Agar, if required.
5 Tryptone 5.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 TOGO M2310 / DSMZ Medium 464a lists 5 g/L Tryptone.

Preparation

  1. Rehydrate and grow lyophilized cells from the ampoule in liquid medium 464; subsequent subculturing may be carried out in liquid medium or an agar medium.
  2. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.319284Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2310
2026-01-28T19:48:09.237137Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.126706Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008897
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.830807+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Reactivation With Liquid Medium 464 (Plate Count Agar)" to "reactivation_with_liquid_medium_464_plate_count_agar" for consistent matching
2026-06-05T07:41:44.091272+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2310_reactivation_464a_score15.py RESOLVED_TOGO_M2310_REACTIVATION_464A_SCORE15 Corrected the TOGO M2310 distilled-water unit to 1 L, added pH 7.0, grounded every DSMZ Medium 464a component, and linked the record as a DSMZ Medium 464a source duplicate.