Overview
- External ontology
- TOGO:M2314 — LB medium
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2314 records LB medium as a one-liter LB wrapper. Sigma-Aldrich L3522 LB Broth (Miller) discloses the Miller formulation: 10 g/L Tryptone, 5 g/L Yeast Extract, and 10 g/L NaCl.
Composition
flowchart LR
CultureMech_008901["lb_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_008901 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_008901 --> ing1_Yeast_extract
ing2_Sodium_chloride["Sodium chloride"]
CultureMech_008901 --> ing2_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing2_Sodium_chloride -.-> CHEBI_26710
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Sigma-Aldrich L3522 LB Broth (Miller) lists 10.0 g/L Tryptone. |
| 2 | Yeast extract | 5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Sigma-Aldrich L3522 LB Broth (Miller) lists 5.0 g/L Yeast extract. |
| 3 |
Sodium chloride
Cl.Na 58.443 g/mol
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Sigma-Aldrich L3522 LB Broth (Miller) lists 10.0 g/L Sodium chloride. |
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Escherichia coli K-12 MG1655
- Term
- Id
- NCBITaxon:511145
- Label
- Escherichia coli str. K-12 substr. MG1655
- Genome assembly id
- GCA_000005845.2
- GCF_000005845.2
- Strain
- K-12 MG1655
- Growth metrics
- Measurement conditions
- LB medium batch culture; source monitored utilization of sugars, alcohols, and organic acids with transcriptional microarrays and associated nutrient utilization with the highest growth rate phase.
- Evidence
- Reference
- PMID:16628448
- Supports
- SUPPORT
- Snippet
- Utilization of these nutrients associated with the highest growth rate of the culture was followed by simultaneous induction of enzymes involved in assimilation of a large group of other carbon sources including D-mannose, melibiose, D-galactose, L-fucose, L-rhamnose, D-mannitol, amino sugars, trehalose, L-arabinose, glycerol, and lactate.
- Explanation
- The article title and abstract directly identify growth of Escherichia coli MG1655 on LB medium. The CultureMech record is LB Miller liquid medium, so this is modeled as exact-parent evidence rather than a variant.
- Is max attainment
- False
- Evidence
- Reference
- PMID:16628448
- Supports
- SUPPORT
- Explanation
- The source title states growth of Escherichia coli MG1655 on LB medium and the abstract discusses the growth-rate phase of that culture.
- Reference
- NCBI:GCF_000005845.2
- Supports
- SUPPORT
- Explanation
- NCBI lists RefSeq assembly GCF_000005845.2 and BioSample SAMN02604091 for Escherichia coli str. K-12 substr. MG1655.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.329102Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2314 |
| 2026-03-13T06:20:01.163677Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008901 |
| 2026-03-17T19:46:19.868774+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB medium" to "lb_medium" for consistent matching |
| 2026-05-05T00:00:00Z | literature_review_manual | ADDED_GROWTH_EVIDENCE | Added strain-specific Escherichia coli K-12 MG1655 growth evidence on LB medium from PMID:16628448. |
| 2026-08-06T00:58:02.675642+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 1 specific strain(s) named, no community signal (#142) |
| 2026-09-11T00:00:00-07:00 | repair_togo_m2314_score15.py | RESOLVED_TOGO_M2314_SCORE15 | TOGO M2314 records LB medium as a one-liter LB wrapper. Sigma-Aldrich L3522 LB Broth (Miller) discloses the Miller formulation: 10 g/L Tryptone, 5 g/L Yeast Extract, and 10 g/L NaCl. |